Tissue Culture Rapid Propagation Method of Zijinniu xinye

[0026] Example 1:

[0027] A method for tissue culture and rapid propagation of the purple golden bull, comprising the following steps:

[0028] A. Integrated cultivation of reproduction and rejuvenation: Disinfect the stems of the purple gold bull with axillary buds and inoculate them into the propagation medium of MS+1.5mg / LBA+0.3mg / L LFS+0.5mg / L IBA to obtain adventitious buds. Cut the adventitious buds into 1.0 cm budded stem segments, spread the budded stem segments flat on the surface of the integrated propagation and rejuvenation medium, and press 1 / 2 of the budded stem segments into the integrated propagation and rejuvenation culture In the base, after the inoculation is completed, transfer to the culture room, first cultivate for 15 days under the conditions of culture temperature 22℃, light intensity 1400lux, light time 8h / d, and then under natural light conditions of culture temperature 25℃ and light intensity 3500lux Continue to cultivate for 15 days to obtain r

[0032] Example 2:

[0033] A method for tissue culture and rapid propagation of the purple golden bull, comprising the following steps:

[0034] A. Integrated cultivation of reproduction and rejuvenation: Disinfect the stems of the purple gold bull with axillary buds and inoculate them into the propagation medium of MS+1.5mg / LBA+0.3mg / L LFS+0.5mg / L IBA to obtain adventitious buds. Cut the adventitious buds into 1.5 cm budded stem segments, spread the budded stem segments flat on the surface of the integrated breeding and rejuvenation medium, and press 1 / 2 of the budded stem segments into the integrated reproduction and rehabilitation culture In the base, after the inoculation is completed, transfer to the culture room, first cultivate for 15 days under the conditions of culture temperature 24℃, light intensity 1400lux, and light time 8h / d, and then under natural light conditions of culture temperature 26℃ and light intensity 4000lux Continue to cultivate for 15 days to obta

[0038] Example 3:

[0039] A method for tissue culture and rapid propagation of the purple golden bull, comprising the following steps:

[0040] A. Integrated cultivation of reproduction and rejuvenation: Disinfect the stems of the purple gold bull with axillary buds and inoculate them into the propagation medium of MS+1.5mg / LBA+0.3mg / L LFS+0.5mg / L IBA to obtain adventitious buds. Cut the adventitious buds into 2.0cm budded stem segments, spread the budded stem segments flat on the surface of the integrated breeding and rejuvenation medium, and press 1 / 2 of the budded stem segments into the integrated reproduction and rehabilitation culture In the base, after the inoculation is completed, transfer to the culture room, first cultivate for 15 days at a culture temperature of 26℃, light intensity of 1400lux, and light time of 8h / d, and then under natural light conditions of culture temperature of 28℃ and light intensity of 4500lux Continue to cultivate for 15 days to obtain ro