Microneedle for treating vitiligo as well as preparation method and application thereof

[0060] A hydrogel for the treatment of vitiligo.

[0061] The preparation method of the hydrogel is as follows.

[0062] 1. Preparation of methacrylylated dextran (DexMA).

[0063] Dissolve 1.0 g of dextran in 10 mL of dimethyl sulfoxide, then add 2.0 g of 4-dimethylaminopyridine, and stir at room temperature for 1 hour; add 3 mL of glycidyl methacrylate to the reaction solution, and stir at room temperature in the dark 48 hours; the reaction solution was collected, dialyzed with a cellulose dialysis bag with a molecular weight cut-off of 3500 for 3 days, and freeze-dried to obtain a product, methacryl dextran.

[0064] Second, the preparation of hydrogels.

[0065] A solution of methacrylylated dextran was prepared with ultrapure water, wherein the mass concentration of methacrylylated dextran was 30%. Add lithium phenyl-2,4,6-trimethylbenzoylphosphonate (LAP) with a concentration of 0.005g / mL to the above solution to make its mass concentration 0.2%, mix, and use 365nm or

[0067] A hydrogel for the treatment of vitiligo.

[0068] The preparation method of the hydrogel is as follows.

[0069] 1. Preparation of methacrylylated dextran (DexMA).

[0070] Dissolve 1.0 g of dextran in 10 mL of dimethyl sulfoxide, then add 2.0 g of 4-dimethylaminopyridine, and stir at room temperature for 1 hour; add 3 mL of glycidyl methacrylate to the reaction solution, and stir at room temperature in the dark 48 hours; the reaction solution was collected, dialyzed with a cellulose dialysis bag with a molecular weight cut-off of 3500 for 3 days, and freeze-dried to obtain a product, methacryl dextran.

[0071] 2. Preparation of host-guest supramolecular HGSM.

[0072] 1. Synthesis of the host molecule ethyl isocyanate modified β-cyclodextrin (β-Cd-AOI 2 ).

[0073] 5 g of β-cyclodextrin (β-CD) was completely dissolved in 50 mL of anhydrous dimethylformamide (DMF) under nitrogen atmosphere. Then, 2 mL of 2-isocyanate ethyl acrylate (AOI) was added dropwise to the mi

[0082] A hydrogel for the treatment of vitiligo.

[0083] The preparation method of the hydrogel is as follows.

[0084] 1. Preparation of methacrylylated dextran (DexMA).

[0085] Same as Example 2.

[0086] 2. Preparation of host-guest supramolecular HGSM.

[0087] Same as Example 2.

[0088] 3. Preparation of hydrogels.

[0089] A mixed solution of methacryl-glucan and HGSM is prepared with ultrapure water, wherein the mass concentration of methacryl-glucan is 30%, and the mass concentration of HGSM is 10%. Add lithium phenyl-2,4,6-trimethylbenzoylphosphonate (LAP) with a concentration of 0.005g / mL to the above mixed solution to make its mass concentration 0.2%, mix, and use 365nm or 405nm illumination , and the irradiation time is 10s-1min to obtain the hydrogel.