The invention discloses a detection method of an antiphospholipid syndrome related antibody. The detection method combines a microsphere analysis technology and a flow analysis technology, and performs joint detection on 12 antiphospholipid antibody indexes such as aCL-IgG, aCL-IgM, aCL-IgA, beta2-GPI-IgG, beta2-GPI-IgM, beta2-GPI-IgA, PS-IgM, PS-IgG, PI-IgM, PI-IgG, PI-IgM, PI-IgG and the like. The detection method comprises the following steps of: 1) preparing a serum sample; 2) preparing and activating microspheres; 3) preparing a capture antibody; 4) carrying out coupling; 5) performing antigen-antibody combination; 6) carrying out fluorescence labeling reaction; and 7) performing flow type detection. Compared with an existing conventional clinical detection method, the detection method has the advantages that joint detection of 12 antiphospholipid marker antibody indexes for diagnosing the anti-phospholipid syndrome is realized, the required sample size is smaller, the operation time is shorter, multi-parameter analysis can be performed, meanwhile, the specificity and the sensitivity are further extremely high, and the detection level and the standardization degree of the anti-phospholipid antibody index are further improved.