The invention discloses a nutritional feed for artificially breeding scorpions and a preparation method of the nutritional feed. The nutritional feed comprises the following raw materials in parts by weight: 12-15 parts of bean pulp, 10-12 parts of earthworms, 5-8 parts of fly larvae, 5-8 parts of tea saponins, 5-6 parts of yolk powder, 4-5 parts of water hyacinth and 3-5 parts of bamboo flavones. The preparation method comprises the following steps: removing the roots of water hyacinth, peeling, cleaning, cutting up, drying, mixing with the bean pulp for co-fermentation, and uniformly mixing a fermented mixture with other raw materials to obtain the nutritional feed. According to the nutritional feed, the earthworms and the fly larvae are substituted for conventional yellow mealworms to serve as a live worm feed; due to the addition of the bean pulp, the yolk powder and the water hyacinth, the nutritional feed is high in protein content, relatively high in water content and relatively rich in nutrition, the food intake amount of the scorpions can be increased, and the disease resistance of scorpion bodies can be improved; meanwhile, due to the addition of tea saponins and bamboo flavones in the feed, the effect of preventing diseases is achieved by the bacterial inhibiting and killing effects of tea saponins and bamboo flavones, so that the use of antibiotics in artificial breeding is effectively avoided.