The invention discloses a Yao medicine preparation for treating acquired immune deficiency syndrome and a preparation method of the Yao medicine preparation. The Yao medicine preparation is composed of an oral preparation and an external washing preparation. The oral preparation comprises the following raw materials in parts by weight: 10-30 parts of longleaf dandelion, 10-30 parts of sophora flower, 10-30 parts of glycyrrhiza uralensis, 10-30 parts of silvervine, 10-30 parts of Cyathea spinulosa stem, 10-30 parts of ardisia japonica blume, 10-30 parts of Mussaenda philippica and 10-30 parts of giant knot weed. The external washing preparation comprises the following raw materials in parts by weight: 20-40 parts of perforate fleeceflower, 20-40 parts of evodia lepta, 20-40 parts of radix sophorae flavescentis, 20-40 parts of entada phaseoloides, 20-40 parts of senecio, 20-40 parts of bayberry bark, 20-40 parts of ninebark, 20-40 parts of silvervine, 20-40 parts of golden cypress skin and 20-40 parts of rhus chinensis. The therapeutic suitable for most infected people of Guangxi is prepared according to traditional Chinese medicine clinical characteristics of Guangxi acquired immune deficiency syndrome and clinical technology solutions for treating of acquired immune deficiency syndrome with traditional Chinese medicine (trial implementation) constituted by State Chinese Medicine Administration Bureau and combining factors of climatic characteristics, environmental factors, personnel conditions, physical characteristics and the like of Guangxi. By utilizing the Yao medicine preparation, symptoms of patients can be relieved, diffusion of human immunodeficiency virus can be restrained, and immunity can be enhanced. The Yao medicine preparation does not have side effects.