The invention discloses a probe composition for detecting
lung cancer gene mutation based on an NGS method and a kit thereof. The probe composition is selected from at least one of probes with
nucleotide sequences as shown in SEQ ID NO.1-75, the kit is suitable for
lung cancer gene mutation detection of FFPE, tissue and
peripheral blood ctDNA based on the NGS method, and then the purposes of earlyscreening of
lung cancer mutant genes, real-time monitoring of recurrence and the like are achieved. The uniquely designed UMI bimolecular tag can effectively reduce
background noise, eradicate tracepollution, remove false positive and ensure the accuracy of a result, so that the sensitivity in ctDNA detection reaches 0.1%. A universal Short-Y joint is used in tissue detection, and the detectionsensitivity can reach 2%. The more possibilities are provided for accurate
targeted therapy of patients. Tissue samples and
plasma samples are similar in
library building
workflow, the simplicity ofthe
workflow is guaranteed, time is saved, the efficiency is high, and the operation is easy.