High water-solubility ruthenium metal complex singlet oxygen fluorescent probe, preparation method thereof, and application thereof

[0050] Embodiment 1, ligand aip-C 4 h 8 -SO 3 Na and the complex [Ru(aip-C 4 h 8 -SO 3 Na) 3 ] Cl 2 Synthesis.

[0051] The synthetic route is as follows:

[0052]

[0053] The basic operation process is as follows:

[0054] 1. Ligand aip-C 4 h 8 -SO 3 Synthesis of Na.

[0055] aip was synthesized according to the literature [M.Mariappan, B.G.Maiya, Effects of anthracene and pyrene units on the interactions of novel polypyridylruthenium(II) mixed-ligand complexes with DNA. Eur. J. Inorg. Chem. 2005, 2164.].

[0056] Under the protection of nitrogen, weigh NaH (0.19g, 7.88mmol, 50% dispersed in mineral oil), wash with redistilled n-hexane three times, add 20mL redistilled DMF, aip (1.2g, 3.03mmol), and heat under reflux for three Hour. After cooling to room temperature, 1,4-butanesulfonyllactone (557mg, 4.24mmol) was added, stirred at room temperature for one hour, then slowly raised to 110°C, and refluxed for 24 hours, during which orange precipitates precipit...

[0063] Embodiment 2, the method and detection performance of complex probe singlet oxygen in buffer solution

[0064] 1. With [Ru(aip-C 4 h 8 SO 3 Na) 3 ] Cl 2 As an example, the method and detection performance of the complex of the present invention as a fluorescent probe for detecting singlet oxygen in neutral and alkaline solutions are introduced.