Synthesis method of compound for protein fluorescent labeling
一种蛋白质荧光、合成方法的技术,应用在化合物的合成领域,能够解决探针连接基团长、亲水端与疏水端分布不均匀等问题,达到毒性小、降低背景荧光干扰、减少距离的效果
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[0015] see Figure 1-Figure 4 , the specific embodiment adopts the following technical scheme: its synthetic method is divided into two steps, and the first step reaction one is to heat and stir the compound I of 1.2-1.5 equivalents and the compound II of 1 equivalent in the acetic anhydride of 1.5-3.5 equivalents for 0.5 -1 hour to obtain the intermediate product III; the second step reaction two is to heat 1 equivalent of the intermediate product III and 1.1-1.5 equivalents of sodium iodide in the organic solvent 2 to 40-70 ° C and reflux for 2-24 hours to obtain the target Product IV, the water-soluble fluorescent compound.
[0016] In the compound I structure, the corresponding cation is potassium ion or sodium ion.
[0017] In the compound II structure, the corresponding cation is potassium ion or sodium ion.
[0018] The organic solvent 1 is a mixed solvent of methanol and chloroform or a mixed solvent of methanol and dichloromethane.
[0019] The advantage of this sp...
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