Kit and method for detecting vibrio parahaemolyticus in aquatic product

A technology for vibrio hemolyticus and aquatic products, which is applied in the fields of biochemical equipment and methods, microbe measurement/inspection, and resistance to vector-borne diseases, etc. It can solve the problems of high false negative rate of frozen food, low bacterial sensitivity, and long culture period To achieve the effect of improving food safety level, simple operation steps and short detection cycle

Inactive Publication Date: 2013-11-27
BOHAI UNIV
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the national standard detection method of Vibrio parahaemolyticus (GB/T 4789.7-2008) is the culture method. After the culture method collects the sample, first enrich the bacteria and culture for 24 hours, conduct preliminary identification according to the shape of the bacterial colony, and then confirm it according to the biochemical identification; Its disadvantages are: long culture cycle, time-consuming and laborious, and low sensitivity to bacteria in the "viable but non-culturable state" (Viable But Non-Culturable, VBNC) that is still pathogenic, which

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0039] Example

[0040] A kit for detecting Vibrio parahaemolyticus in aquatic products, including sterile deionized water, PCR reaction solution, Taq DNA polymerase, ethidium bromide DNA dye, bromophenol blue loading buffer, standard substance, reference substance;

[0041] Among them, the PCR reaction solution contains PCR reaction buffer, MgCl 2 , Deoxyribonucleoside triphosphates (dNTPs), primers for detection;

[0042] The detection primers are a mixture of the following 4 primers (Table 1).

[0043] Table 1

[0044]

[0045] The detection primers used the D-type amino acid dehydrogenase small subunit gene of Vibrio parahaemolyticus as template DNA, designed with Primer Premier 5, and synthesized by Shenggong Bioengineering (Shanghai) Co., Ltd.;

[0046] The standard product is a recombinant plasmid containing the amplified product obtained after PCR amplification using the Vibrio parahaemolyticus genome as template DNA and P3 and P4 as primers, which is constructed by the following

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a kit and method for detecting the vibrio parahaemolyticus in an aquatic product. The kit comprises sterile deionized water, PCR (Polymerase Chain Reaction) liquid, TaqDNA (Deoxyribonucleic Acid) polymerase, an ethidium bromide DNA dye, a bromophenol blue loading buffer, a standard sample and a control sample. The detection method comprises the following steps: firstly collecting samples, carrying out enrichment culture on the samples, extracting the DNAs after the enrichment culture, preparing PCR reaction tubes, carrying out PCR amplifications on the extracted DNAs on a PCR instrument respectively to obtain PCR amplification products, and judging the detection results. The kit has the following advantages that the kit can be applied to the aspects of the fast detection of the vibrio parahaemolyticus, the epidemiological investigation, the drug efficacy monitoring and the like. Compared with an existing detection method, the method disclosed by the invention is convenient to use, good in reliability, short in detection period, high in sensitivity, strong in specificity, low in cost, simple in operation step and suitable for high throughput operation and standard operation, and is of great significance in improving the safety level of food.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Owner BOHAI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap