Tissue culture method of culturing erianthus arundinaceus by employing bacteriostatic culture medium

A medium and medium preparation technology, applied in the biological field, can solve the problems of high manual operation cost and high energy consumption, and achieve the effects of reducing tissue culture costs, simplifying tissue culture procedures, and simple operation.

Inactive Publication Date: 2017-06-13
FUJIAN AGRI & FORESTRY UNIV
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AI Technical Summary

Benefits of technology

This patented technology allows for better control over microorganisms during culturing processes while maintaining effective levels of anti-bactility agents without requiring expensive specialized facilities like autoclaving at very low temperatures. It provides an economically viable solution that eliminates environmental concerns associated with traditional methods.

Problems solved by technology

This patented technical solution described involves creation of new varieties called Bignonia cathayana (L.) from natural sources like Sugar Cane Erisiaceae. These types include two main genera: One being African American type A/Brixium citrinosulense rootstock and another producing sweet betel flavorings during feral reproductive cycles. They require less space than traditional ways but they also provide benefits over time due to their unique properties including resistence against weather conditions, tolerance towards disease vectors, and fast reproduction rates when compared to existing techniques involving pollinators.

Method used

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  • Tissue culture method of culturing erianthus arundinaceus by employing bacteriostatic culture medium

Examples

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Embodiment 1

[0033] Embodiment one: a kind of tissue culture method utilizing bacteriostatic medium to cultivate Banmao

[0034] A method for tissue culture utilizing antibacterial medium to cultivate Banmao, comprising the following steps:

[0035] 1. Disinfection of the culture container: Soak the culture bottle, bottle cap and plastic plate for inoculation in an aqueous solution of 100 mg / L sodium hypochlorite + 10 mg / L cycloserine + 0.5 mg / L sodium dodecylsulfonate for no less than 10 hours. Save for later use;

[0036]2. Medium preparation: induction medium is MS+4.0mg / L 2,4-D+60mg / L sodium hypochlorite+1.0mg / L cycloserine+30mg / L Timentin+0.3mg / L dodecyl sulfonate Sodium Acetate+30g / L Sucrose+3.0g / L Agar Powder, pH5.8; Differentiation and Proliferation Medium: MS+1.0mg / L IBA+1.0mg / L KT+60mg / L Sodium Hypochlorite+1.0mg / L Ring Serine+30mg / L Timentin+0.3mg / L Sodium Dodecyl Sulfonate+30g / L Sucrose+3.0g / L Agar Powder, pH5.8; Rooting medium is 1 / 2MS+3.0m

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Abstract

The invention relates to a tissue culture method for cultivating Pseudomonas chinensis by using an antibacterial medium, which comprises the steps of disinfection of culture containers, preparation of culture medium, preparation of sterile water, induction culture, differentiation and proliferation culture, rooting culture and bottle seedling transplanting. During the bacteriostatic tissue culture process of P. chinensis of the present invention, both the culture container and the culture medium do not need to be sterilized under high temperature and high pressure, which reduces the workload and energy consumption, simplifies the technical links of the P. chinensis tissue culture, and reduces the tissue culture cost of the P. chinensis; and The operation is simple, and it can be used only after being prepared according to different medium formulas, and has strong practicability and good popularization. Compared with the conventional tissue culture method of P. chinensis of the present invention, the method for inhibiting bacteria of P. chinensis can reduce the cost by more than 10%.

Description

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Claims

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Application Information

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Owner FUJIAN AGRI & FORESTRY UNIV
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