Preparation method of chitosan-phenolic acid conjugate

[0020] A kind of preparation method of chitosan-vanillic acid conjugate, concrete steps are as follows:

[0021] 1. Take a three-necked flask with a clean volume of 5L, add 2L of acetic acid with a volume fraction of 2%, add 10g of chitosan and dissolve it, then add 20mL of 1mol / L H containing 1.08g of ascorbic acid dropwise. 2 o 2 , and then filled with nitrogen for 30 minutes.

[0022] 2. Dissolve 10 g of vanillic acid in 100 mL of ethanol to obtain a vanillic acid solution, add the vanillic acid solution to the reaction system in step 1, seal the flask mouth with plastic wrap, and react at 25°C for 24 hours under electromagnetic stirring.

[0023] 3. The reaction solution was dialyzed against distilled water for 72 hours with a 14000Da molecular weight cut-off membrane to remove unreacted vanillic acid, and the obtained dialysate was dried by a spray dryer (inlet temperature 140°C, outlet temperature 77°C) to obtain chitosan-vanilla acid conjugates.

[0025] A kind of preparation method of chitosan-coumaric acid conjugate, concrete steps are as follows:

[0026] 1. Take a three-necked flask with a clean volume of 5L, add 2L of acetic acid with a volume fraction of 2%, add 10g of chitosan and dissolve it, then add 20mL of 1mol / L H containing 1.08g of ascorbic acid dropwise. 2 o 2 , and then filled with nitrogen for 30 minutes.

[0027] 2. Dissolve 10 g of coumaric acid in 100 mL of ethanol to obtain a coumaric acid solution, add the coumaric acid solution to the reaction system in step 1, seal the flask mouth with plastic wrap, and react at 25°C for 24 hours under electromagnetic stirring.

[0028] 3. The reaction solution was dialyzed against distilled water for 72 hours with a 14000Da molecular weight cut-off membrane to remove unreacted coumaric acid, and the obtained dialysate was dried by a spray dryer (inlet temperature 140°C, outlet temperature 77°C) to obtain chitosan- Coumaric acid conjugates.

[0030] A kind of preparation method of chitosan-ferulic acid conjugate, concrete steps are as follows:

[0031] 1. Take a three-necked flask with a clean volume of 5L, add 2L of acetic acid with a volume fraction of 2%, add 10g of chitosan and dissolve it, then add 20mL of 1mol / L H containing 1.08g of ascorbic acid dropwise. 2 o 2 , and then filled with nitrogen for 30 minutes.

[0032] 2. Dissolve 10 g of ferulic acid in 100 mL of ethanol to obtain a ferulic acid solution, add the ferulic acid solution to the reaction system in step 1, seal the flask mouth with a plastic wrap, and react at 25°C for 24 hours under electromagnetic stirring.

[0033] 3. The reaction solution was dialyzed against distilled water for 72 hours with a 14000Da molecular weight cut-off membrane to remove unreacted ferulic acid, and the obtained dialysate was dried by a spray dryer (inlet temperature 140°C, outlet temperature 77°C) to obtain chitosan- Ferulic acid conjugates.