ORF074 (Open Reading Frame 074) gene deleted strain of infectious spleen and kidney necrosis virus as well as preparation method and application thereof
一种脾肾坏死病毒、ORF074的技术,应用在传染性脾肾坏死病毒ORF074基因缺失株及其制备领域,能够解决研究未有报道等问题,达到免疫率高、节省成本、免疫方式便捷的效果
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[0052] The following description is a preferred embodiment of the present invention, it should be pointed out that for those skilled in the art, without departing from the principle of the present invention, some improvements and modifications can also be made, and these improvements and modifications are also considered Be the protection scope of the present invention.
[0053] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The experimental method that does not indicate specific condition in the embodiment, generally according to routine condition. Unless otherwise specified in the examples of the present invention, all reagents and consumables used are commercially available.
[0054] The embodiment of the present invention provides a preparation method of recombinant virus vaccine with ORF074 gene deletion of infectious spleen and kidney necrosis virus, comprising the following steps:
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