Rapid decomposing microbial agent as well as preparation method and application thereof

[0059] The separation and purification, screening and identification of embodiment 1 yeast Y-1

[0060] 1) Separation and purification: Accurately weigh 10g of distiller's grains sample from the natural accumulation and fermentation of Wuliangye Group in Yibin City, Sichuan Province, add it to a sterilized triangular flask with glass beads containing 100mL of cooled sterile water, and shake it on a shaker at 200r / min Mix evenly for 30 minutes, let it stand for 5 minutes, absorb 1 mL of the supernatant, and serially dilute to obtain 10 -1 -10 -5 Concentration, select 10 -1 、10 -2 、10 -3 、10 -4 、10 -5Use a pipette to absorb 100 μL of each gradient suspension, and spread it evenly on the YPD plate with streptomycin sulfate with a spreader. The final concentration of Streptomyces sulfate in the medium is 30 μg / mL. For bacterial water control, place the plate upside down in the incubator and incubate at 30°C for 7 days. Each treatment is set up to repeat 3 groups horizontall

[0100] Example 2 Low pH Tolerance Test of Saccharomyces fornicata Y-1

[0101] (1) Method: Pick an appropriate amount of yeast Y-1 and insert it into the YPD liquid medium, and culture it with shaking at 180 rpm at 30°C for 48 hours to obtain the seed liquid. pH value (1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5) in the Erlenmeyer flask of sterilized YPD liquid medium, each pH value was repeated three times, and those without inoculation were Blank control, 30°C, 180r / min shaking culture for 48h, observe whether the bacterial liquid in the Erlenmeyer flask is turbid and turbid at each pH, ​​and measure the bacterial activity of the turbid bacterial liquid at the same time;

[0102] (2) Test results: Except for the pH values ​​of 1.0, 1.5, 2.0 and the CK group bacteria liquids were still clear, the YPD liquid culture media with different pH (2.5-6.5) were all turbid, indicating that S. It can grow within the range of pH 2.5-6.5, and the bacteria suspension with differ

[0103] Embodiment 3 solid-state fermentation of Saccharomyces fornicata Y-1

[0104] (1) Strain activation: use an inoculation loop to pick an appropriate amount of Saccharomyces fumigatus Y-1, streak it onto the PDA plate medium, and culture it at 30°C for 48 hours;

[0105] (2) Preparation of the primary seed liquid: pick an appropriate amount of activated Saccharomyces fornicosa Y-1 with an inoculation loop and inoculate it in the YPD liquid medium, and culture it with shaking at 200 rpm at 30°C for 24 hours to obtain the primary seed liquid;

[0106] (3) Preparation of the secondary seed liquid: inoculate the primary seed liquid into the YPD liquid medium again according to the inoculum amount of 1% (v / v), culture at 30° C. with shaking at 180 rpm for 36 hours, and obtain the secondary seed liquid;

[0107] (4) Solid-state fermentation research:

[0108] ① Mixing material: according to the formula of solid fermentation medium A, first use 500mL tap water to mix glucose, pe