Method for extracting saponin active ingredients from bark of acacia mearnsii de willd based on deep eutectic solvents

[0031] Extraction of Total Saponins from Black Wattle Bark

[0032] The preparation method of the aqueous solution of DESs: heating choline chloride and citric acid under normal pressure at 100° C. for 2-4 hours and synthesizing DESs under continuous stirring until a stable uniform liquid is formed. All prepared DESs were cooled to room temperature and dried in a vacuum oven at 50 °C for 24 h to obtain DESs. Dissolving DESs in water at 30% by volume to obtain a DESs aqueous solution.

[0033]Take 3 parts of 0.5g (accurate to 0.001g) black wattle bark powder (through a 20 mesh sieve) raw material in a 25mL conical flask, add a volume fraction of 30% choline chloride-lemon Acidic DESs aqueous solution, treated at 55°C for 20 minutes, suction filtered, and the filtrate obtained is the active ingredient of saponins.

[0034] The filtrate was transferred to a 100mL volumetric flask, and the 30% ethanol solution was adjusted to 100mL for subsequent determination.

[0042] The preparation method of the aqueous solution of DESs: heating choline chloride and glucose under normal pressure at 100° C. for 2 to 4 hours and synthesizing DESs under continuous stirring until a stable uniform liquid is formed. All prepared DESs were cooled to room temperature and dried in a vacuum oven at 50 °C for 24 h to obtain DESs. Dissolving DESs in water at 30% by volume to obtain a DESs aqueous solution.

[0043] Extraction of Total Saponins from Black Wattle Bark

[0044] Take 3 parts of 0.5g (accurate to 0.001g) black wattle bark powder raw material (through 20 mesh sieve) respectively in 25mL Erlenmeyer flask, add volume fraction as 30% choline chloride-glucose DESs with solid-liquid ratio 1g:15mL The aqueous solution was treated at 60°C for 25 minutes, filtered, the filtrate was transferred to a 100mL volumetric flask, and the volume of 30% ethanol solution was adjusted to 100mL for subsequent determination.

[0046] The preparation method of the aqueous solution of DESs: heating choline chloride and glucose under normal pressure at 100° C. for 2 to 4 hours and synthesizing DESs under continuous stirring until a stable uniform liquid is formed. All prepared DESs were cooled to room temperature and dried in a vacuum oven at 50 °C for 24 h to obtain DESs. Dissolving DESs in water at 30% by volume to obtain a DESs aqueous solution.

[0047] Extraction of Total Saponins from Black Wattle Bark

[0048] Take 3 parts of 0.5g (accurate to 0.001g) black wattle bark powder raw material (over 20 mesh sieve) respectively in a 25mL conical flask, and add a volume fraction of 30% choline chloride-glucose DESs with a solid-liquid ratio of 1g:10mL The aqueous solution was treated at 55°C for 25 minutes, filtered, the filtrate was transferred to a 100mL volumetric flask, and the volume of 30% ethanol solution was adjusted to 10mL for subsequent determination.

[0049] Taking oleanolic acid as a stand