Production process for separation and purification of beta-glucan in oat bran based on ultrafiltration membrane technology

An oat bran, separation and purification technology, applied in membrane technology, semi-permeable membrane separation, ultrafiltration and other directions, can solve the problems of industrial production and large-scale clinical application limitations, increase production costs, and long production cycles, and achieve single High separation efficiency, easy extraction and easy operation

Pending Publication Date: 2020-04-07
植萃优品(天津)生物科技有限公司
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  • Claims
  • Application Information

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Problems solved by technology

[0004] Therefore, in order to meet the needs of developing β-glucan, a high-efficiency extraction and separation technology is needed, and the traditional techniques for separating and extracting β-glucan from natural products in the prior art, such as water extraction and alcohol analys

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[0029] The present invention will be described in further detail below through specific examples. The following examples are only descriptive and not restrictive, and the protection scope of the present invention cannot be limited by this.

[0030] A production process for separating and purifying β-glucan from oat bran based on ultrafiltration membrane technology. Oat bran crushing → β-glucan extraction → preparation of β-glucan aqueous solution → microfiltration → ultrafiltration → freeze drying →The specific process steps of β-glucan products are as follows:

[0031] ⑴Oat bran crushing: the raw oats are crushed and the bran is screened, and the mesh size is 60-120;

[0032] ⑵ β-glucan extraction: take a buffer solution with a pH of 4-6 and α-amylase, the ratio of bran to material-liquid is 1:80-100, extraction temperature is 80-90℃, extraction is 5-6h, after cooling to normal temperature , Adjust the pH to 6-8, centrifuge to take the supernatant, alcohol precipitation and centrifuga

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Abstract

The invention relates to a production process for separation and purification of beta-glucan in oat bran based on ultrafiltration membrane technology. The process includes: crushing oat bran, extracting beta-glucan, preparing a beta-glucan aqueous solution, carrying out microfiltration, performing ultrafiltration, and conducting freeze drying to obtain a beta-glucan finished product. The innovation point of the process lies in that ultrafiltration technology is utilized to realize efficient extraction, separation and purification, and compared with the traditional separation processes like rectification, extraction, recrystallization, adsorption, etc., the process has the advantages of low energy consumption, high single-stage separation efficiency, simple equipment, no pollution and the like. The process has extremely high beta-glucan separation efficiency and low active ingredient destruction rate, is easy to operate, and only needs to control the operation pressure, the flow speed and the filtrate concentration, and for unstable beta-glucan with relatively large molecular weight, wide distribution range and large solution viscosity, the process is a separation means with great development potential.

Description

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Claims

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Application Information

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Owner 植萃优品(天津)生物科技有限公司
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