Bio-control inoculant for inhibiting streptococcus agalactiae as well as preparation method and application of bio-control inoculant

[0035] Example 1 - Antibacterial effect of boronine binder fermentation

[0036] The inhibitory effect of Boride Bacillus Bacillus Bacillus Bacillus Bacillus was used to determine the inhibitory effect of Boriocide glycide growth.

[0037] Experimental group 1:

[0038] Experimental group 1-1:

[0039] Seed culture fluid includes the following components: 5 g / l yeast extract, 10 g / L - panazolic protein and 10 g / L sodium chloride, pH 7.0;

[0040] The proliferation culture medium includes the following components: 5.2 g / l yeast extract, 10.5 g / L of the pancreatine, 10.5 g / l sodium chloride and 2.8 g / L Polyglutamic acid, pH 7.0;

[0041] Fermentation culture fluid includes the following components: 5.2 g / l yeast extract, 10.5 g / L of the pancreatine, 10.5 g / l sodium chloride, 1.2 g / l polyglutamic acid and 0.3 g / l rock white dish, pH Value 6.8; assigns a seed culture solution, a proliferation culture solution, and a fermentation culture med

[0073] Example 2 - Prevention and treatment of roclasty bacteria

[0074] A bioplasmic agent for suppressing a variable bacteria was composed of boronine bonosporium bonosporium bonosporium bonosporium bonosporine, 10 parts water, and 14 mooyi protein powder prepared from 30 experimental group 1-1;

[0075] The preparation method of the bioplastic bacteria is: mixed with b bedium bonosporium bonosporium bonosporium bonosporium, mixed with kaolin, stir, spray dry, pulverized, and obtained biopsies;

[0076] Choose a healthy tilapia 40 tail, weigh 80-90g, divided into 2 groups, each set of 20 tails, feed the strike feed 2 times a day, change the water every 5D, regulate the water, and The agent is mixed with tilapia feed 1: 200 mixed into the test group, and the fluorine tissue is mixed with tilapia 1: 2000 mixed as a pharmaceutical group; after feeding 10 days, 0.2 ml of the dose of 0.2ml in the strip of the strike Uppermptogenic bacteria suspension, continued to feed 7d, observe the