Anti-podoplanin antibody, and pharmaceutical composition containing Anti-podoplanin antibody
Active Publication Date: 2012-12-06
ZENOGEN PHARM CO LTD
View PDF3 Cites 2 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Benefits of technology
[0092]According to the present invention, an anti-podoplanin antibody having an effector activity such as ADCC activity or CDC activity can be obtained. The anti-podoplanin antibody having an effector activity is capable of specifically inhibiting not only hematogenous metastasis of cancer, but also growth of tumor cells with high expression of podoplanin. Therefore, the anti-podoplanin antibody having an effector activity is effectively used for the treatment of brain tumor, mesothelioma, testicular tumor, ovarian cancer, and a variety of squamous cancers (oral cancer, pharynx cancer, larynx cancer, esophageal cancer, lung cancer, skin cancer, and uterine cervical cancer), from each of which the expression of podoplanin is observed.
[0093]Further, the human chimeric antibody in accordance with the present invention has a remarkably high effector activity and is also considered to exhibit low antigenicity with respect to a human, and is therefore useful as a safe and highly effective pharmaceutical product.
Problems solved by technology
On the other hand, it has been believed that platelet aggregation accelerates the adhesion of cancer cells to vascular endothelial cells, and the release of growth factors results in local proliferation of cancer cells.
Further, since ADCC activity or CDC activity is dependent on subclasses of an antibody, it cannot be said that the antibody, even when having ADCC activity, has CDC activity and it cannot be said that the antibody, even when having CDC activity, has ADCC activity.
However, such methods do not always bring about enhancement of ADCC activity.
Further, even when ADCC activity is enhanced, since there is a variety of embodiments in modification of an amino acid sequence or control of a sugar chain structure, it is not easy to find a method of enhancing ADCC activity for a given antibody.
However, with regard to the method for preparing a chimeric antibody or a humanized antibody, there is no standardized method which is universally applicable to any antibody.
Even when a chimeric antibody is constructed based on the antibody obtained from a different species, the resulting chimeric antibody may lose both a binding activity and an effector activity.
Further, where a monoclonal antibody constructed with rodents such as mice or rats is made to be a chimeric antibody or a humanized antibody, it cannot be guaranteed that an equivalent activity can be obtained and an antibody having low antigenicity can be obtained.
Further, an amino acid sequence of NZ-1 antibody, an amino acid sequence of a CDR or a gene sequence encoding the same has not been elucidated and there is no example specifically demonstrating a design of chimeric antibodies or the like.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Example
Anti-Podoplanin Antibody)
[0112]The present invention provides an anti-podoplanin antibody for which an epitope is a polypeptide consisting of the amino acid sequence set forth in SEQ ID NO:1 (excluding rat NZ-1 antibody having a heavy chain consisting of the amino acid sequence set forth in SEQ ID NO:2 and a light chain consisting of the amino acid sequence set forth in SEQ ID NO:3, hereinafter, also referred to as “anti-podoplanin antibody of the present invention”).
[0113]The anti-podoplanin antibody of the present invention is a novel antibody, inhibits binding of podoplanin to CLEC-2, has high effector activity such ADCC activity or CDC activity, and has a tumor growth-inhibitory potency (which will be described hereinafter).
[0114]There has been known hitherto that rat NZ-1 antibody having a heavy chain consisting of the amino acid sequence set forth in SEQ ID NO:2 and a light chain consisting of the amino acid sequence set forth in SEQ ID NO:3 inhibits binding of podoplanin to CLEC
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more PUM
| Property | Measurement | Unit |
|---|---|---|
| Fraction | aaaaa | aaaaa |
| Cytotoxicity | aaaaa | aaaaa |
| Inhibition | aaaaa | aaaaa |
Login to view more
Abstract
Disclosed are: an anti-podoplanin antibody which has a high binding activity and a high effector activity and has low antigenicity in humans or mice; and others. Specifically disclosed are: a chimeric antibody comprising an anti-podoplanin antibody for which an epitope is a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:1 (excluding rat NZ-1 antibody having a heavy chain comprising the amino acid sequence set forth in SEQ ID NO:2 and a light chain comprising the amino acid sequence set forth in SEQ ID NO:3), and a heavy chain complementarity determining region and a light chain complementary determining region of the anti-podoplanin antibody; and others.
Description
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more Application Information
Patent Timeline
Login to view more Owner ZENOGEN PHARM CO LTD
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Try Eureka
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap