Compositions containing combinations of nitrogen-fixing bacteria and additional agents and their use in fixing nitrogen in plant species

Treatment of Growing Maize Plants with a Composition of the Present Invention: Field Trial 1

Method

[0122]The trial was sown on the format date 29 of Aug. 2017 using the pioneer maize silage variety number 32-W-68. The field was washed from possible nitrogen using sprinklers irrigation. The combination treatments administered to the plants which are of relevance for the present study were:[0123]C=Untreated Control without nitrogen.[0124]F=Rhizobium leguminosarum biovar viceae, 1% emulsion diluted by 20 and Calirus 1% sprayed in the sowing trench.[0125]H=Rhizobium leguminosarum biovar viceae, 1% emulsion diluted by 20 and Calirus 1% sprayed in the sowing trench+granules containing the emulsion and Bacillus s. 0.5% (granule-B).

[0126]The amount of the solution containing the Rhibozium 1%, the activating agent emulsion and the fertilizer (Calirus) (treatments F and H) was calculated such that 2 liters per 1000 m row were added to the sowing trench.

[0127]In the case of treatment H, the gra...

Treatment of Growing Maize Plants with a Composition of the Present Invention: Field Trial 2

Methods

[0146]As in the case of the field study reported in Example 1, above, this trial was sown on the 29 of Aug. 2017 using the pioneer maize silage variety number 32-W-68. The field was washed from possible nitrogen using sprinklers irrigation. The combination treatments administered to the plants which are of relevance for the present study were:[0147]1. Rhizobium leguminosarum biovar viceae, 1% sprayed in the sowing trench and Granules containing the activating agent emulsion together with Calirus and Rhizobium 0.5%.[0148]2. Rhizobium leguminosarum biovar viceae, 1% and the activating agent emulsion sprayed in the sowing slot, and Granules containing the activating agent emulsion together with Calirus and Rhizobium 0.5%.

[0149]The amount of the solution containing either the Rhibozium 1% (treatment 1) or Rhizobium 1%, and activating agent (treatment 2) was calculated such that 2 liters pe...

Treatment of Growing Maize Plants with a Composition of the Present Invention: Field Trial 3—Growth Parameters

Methods

[0170]This trial was sown during the Israeli growing season of 2018 using the pioneer maize silage variety number W86. The field was washed from possible nitrogen using sprinklers irrigation. The combination treatments administered to the plants which are of relevance for the present study were:[0171]A. Positive Control—full commercial nitrogen. The plants were treated with 30 units of nitrogen per 1000 m2 by means of applying to this area 60 kg urea containing 46% urea.[0172]B. Negative Control—no added nitrogen.[0173]C. Rhizobium leguminosarum biovar viceae, 3% (containing 109 organisms) was added to the activating agent emulsion described in ‘general methods’ hereinabove, to which Calirus (1%) was also added. Perlite granules were soaked with this mixture as described hereinabove. The granules were added to the sowing trench at a density of 2 Kg granules per 1000m2...