Disclosed is a helium leakage detection device and method for a double-layer pipe with an interlayer. In a helium leakage detection tool for a double-layer pipe, an inner pipe is sleeved with an outerpipe, the inner pipe is connected with the first end of a sealing flange box in a sealed mode through a first sealing piece, the outer pipe is connected with the second end of the sealing flange boxthrough a second sealing piece, and the diameter of the first end is smaller than that of the second end; one end of the inner pipe is blocked by a first blocking plate, and the other end of the innerpipe is blocked by a second blocking plate; a helium introducing hole is formed in the first blocking plate; an inflation cover is arranged outside the outer pipe, and a helium introducing hole is formed in the inflation cover. The detection device is simple in structure, convenient to disassemble and assemble, capable of achieving air tightness testing of the inner layer and the interlayer of the double-layer pipe, high in leakage detection precision, high in detection efficiency, low in cost, capable of achieving overall testing and capable of meeting the leakage detection requirement of the double-layer pipe with the interlayer.