7 results about "Hybrid seed" patented technology

The invention discloses primers and a method for purity identification of hybrid seeds of Chinese pumpkin 'Guangmi NO.1'. The primers are VRISSRPMK8-F:5'-TAATTAAGGCGTGAAATTGG-3' and VRISSRPMK8-R:5'-GAAAAGAAACATAAACAGGGC-3'. The identification method is as follows: 1) extracting a genome DNA (deoxyribonucleic acid) of a pumpkin seedling; 2) carrying out PCR (polymerase chain reaction) amplification by taking the genome DNA of the pumpkin as a template and using the SSR primers VRISSRPMK8; 3) carrying out gel electrophoresis on the amplified product; and 4) analyzing the electrophoresis result, wherein only the single plant simultaneously having parent specific bands is a real hybrid, and the plant lacking of any one band is marked as false hybrid. The primers and the method can be used for effectively distinguishing the hybrid seed of the 'Guangmi NO.1' from the female parent and the male parent, and can identify the purity of the hybrid seeds rapidly and accurately. The method has the advantages of being fast and accurate, low in cost and simple in operation, and the like.

The invention discloses a method for comprehensively adjusting and controlling florescence in seed production of cauliflower by hybridization, pertaining to the technical field of seed production of crops by hybridization. The method comprises the steps of (1) selecting planting areas, (2) selecting seeding time, (3) physical control and (4) chemical control. In the method of seed production by hybridization of the invention, early seasonal, middle seasonal and serotinous cauliflower varieties are selected respectively according to different elevations of 1000m, 1200m and 1400m and different seeding time of July, August and September; different fur cutting and ball cutting treatments and water and fertilizer control are adopted according to the growth speed of the ball flowers; technical measures such as spraying with GA with the concentration of 30ppm or Kaihuajin solution with the concentration of 500 times are applied to the varieties that are slow in blooming and bolting in the same group after the ball cutting is applied to the varieties. The method of the invention can ensure that the respective florescence of parents is staggered within 20 days in the seed production of crossbred F1 of the matched early seasonal, middle seasonal and serotinous cauliflowers and can ensure the connecting of the florescence by means of artificial regulation, and the yield of the seed production is 41.7 kg/mu higher than that of the inexperimental group.

The invention provides a screening method of high-oil high-oleic-acid salt-tolerant peanuts, and belongs to the technical field of new peanut variety cultivation. According to the method, high-oil peanuts are used as female parents, high-yield high-oleic-acid peanuts are used as male parents, and hybridization is performed; hybrid detection is performed on F1-generation seeds obtained after hybridization, true hybrids are screened, and false hybrids are eliminated; the F1 seeds of the true hybrid seeds are sowed in a field, and selfed to obtain F2-generation legume seeds; the oil content and the oleic acid content of the F2-generation seeds are detected by using a near infrared instrument in a non-destructive manner, and high-oil high-oleic acid seeds with the oil content of 55% or above and the oleic acid content of 75% or above are screened; salt-tolerant peanuts are used as male parents and high-oil high-oleic-acid F2-generation seeds obtained by screening are used as female parents, composite hybridization is carried out to obtain composite cross F1 pod seeds; and multiple cross is carried out on the F1-generation seeds, hybrid detection, screening true hybrids, eliminating false hybrids and the like are carried out. According to the screening method, the high-oil high-oleic-acid salt-tolerant peanut variety can be obtained.

The invention discloses a sunflower hybrid pollination breeding method under southern humid and hot conditions. The method comprises the following steps: selecting a land which is relatively flat in terrain, medium or above in fertility and good in drainage; selecting full seeds, and drying the seeds in the sun for 2-3 days before planting so as to enhance the activity of enzymes in the seeds and improve the germination potential and the germination rate; generally applying 0.5-1.0 ton/mu of organic fertilizer, 10-15 kg/mu of calcium magnesium phosphate fertilizer, 10-15 kg/mu of urea and 20-30 kg/mu of potassium sulfate type compound fertilizer as base fertilizer; and adopting a seedling transplanting or breathing type precision dibbler direct sowing mode, and carrying out spring sowing, film mulching and weed suppression, wherein the planting row spacing is 50 * 55 cm, and the planting spacing is 45 * 50 cm in autumn. According to the scientific sunflower hybrid seed production method, through the technical means of land selection and preparation, seed selection, fertilization, planting and the like, the sunflowers are hybridized, so that the disease and pest resistance of the sunflowers is enhanced; and the cultivated sunflower variety is suitable for being planted in southern humid and hot conditions, and has the advantages of non-high susceptibility to other diseases, high-temperature heat damage resistance, excellent comprehensive agronomic characters and wide adaptability.