The invention relates to antibacterial liquid soap for washing hands in a surgical operation and a preparation technology of the antibacterial liquid soap. The antibacterial liquid soap is prepared from the following components in parts by weight: 20-30 parts of green grape seed oil, 120-150 parts of peony seed oil, 0.5-1.0 part of grapefruit extract, 1.5-2.5 parts of oleuropein, 1.0-2.0 parts of chlorhexidine gluconate, 0.5-1.0 part of natural wild chrysanthemum flower extract, 2.2-3.0 parts of potassium hydroxide, 0.5-3.0 parts of sodium cocoyl acid isethionate, and the balance of water. The antibacterial liquid soap does not contain phosphates, has the natural features, is free of irritation to the skin, is safe and reliable, belongs to an ultralow-foaming washing product, and is particular suitable for rinsing; a special calcium soap dispersing agent is specially added, so that the detergency of the antibacterial liquid soap is strong; the natural wild chrysanthemum flower extracting liquid is added, so that the antibacterial liquid soap is refreshing and comfortable, moist, mild, and rich in aroma; after the antibacterial liquid soap is used for washing hands, after-fragrance is left on the hands; and the antibacterial liquid soap has the obvious bacteriostasis for staphylococcus aureus and candida albicans.