Method for synchronous mixed cultivation of oil-containing microorganism

[0030] Example 1

[0031] Rhodotorula glutinis and Chlorella vulgaris were separately cultured in shake flasks using their respective culture media, and after 2 days of culture, seed solutions with OD values ​​of 5 and 10 were obtained. Add the cultured Rhodotorula glutinosa seed liquid, chlorella seed liquid and mixed medium to the mixing tank for mixing. The volume ratio of the Rhodotorula glutinosa seed liquid to the chlorella seed liquid is 1:1, and the seed liquid and mixed culture The volume ratio of the base is 1:5, and the pH of the mixture is adjusted to 7.0. Inoculate the above-mentioned mixed solution into a 10L photobioreactor according to the filling amount of 8L. The reactor is an air-lift stirring type, which can realize the back-mixing of the culture solution. The reactor is a vitreous body. The fluorescent light source is set and the light switch is automatically controlled. Time, the conversion of light to dark during the formation of chlorella culture. The cultu

[0032] Example 2

[0033] As described in Example 1, Rhodotorula glutinis and Chlorella vulgaris were separately cultured in shake flasks using their respective culture media. After 2 days of culture, seed solutions with OD values ​​of 15 and 2 were obtained. Add the cultured Rhodotorula glutinosa seed liquid, chlorella seed liquid and mixed medium to the mixing tank for mixing. The volume ratio of the Rhodotorula glutinosa seed liquid to the chlorella seed liquid is 1:5, and the seed liquid and mixed culture The volume ratio of the base is 1:10, and the pH of the mixture is adjusted to 6.5. Inoculate the above-mentioned mixed solution into a 10L photobioreactor according to the filling amount of 8L. The reactor is an air-lift stirring type, which can realize the back-mixing of the culture solution. The reactor is a vitreous body. The fluorescent light source is set and the light switch is automatically controlled. Time, the conversion of light to dark during the formation of chlor

[0034] Example 3

[0035] As described in Example 1, Rhodotorula glutinis and Chlorella vulgaris were separately cultured in shake flasks using their respective culture media. After 2 days of culture, seed solutions with OD values ​​of 10 and 6, respectively, were obtained. Add the cultivated Rhodotorula glutinis seed liquid, chlorella seed liquid and mixed medium to the mixing tank for mixing. The volume ratio of the Rhodotorula glutinosa seed liquid to the chlorella seed liquid is 1:10, and the seed liquid and mixed culture The volume ratio of the base is 1:20, and the pH of the mixture is adjusted to 7.5. Inoculate the above-mentioned mixed solution into a 10L photobioreactor according to the filling amount of 8L. The reactor is an air-lift stirring type, which can realize the back-mixing of the culture solution. The reactor is a vitreous body. The fluorescent light source is set and the light switch is automatically controlled. Time, the conversion of light to dark during the