Preparation method of medicine-carrying temperature-sensitive self-assembly lipidosome

[0028] Take by weighing the phosphatidylcholine of 0.05g, be dissolved in 5ml chloroform, add the ketoprofen of 0.5g poly-N-isopropylacrylamide and 0.006g, stir at room temperature on the magnetic stirrer, get containing medicine temperature. Sensitive polymer electrospray stock solution.

[0029] The prepared electrospray stock solution was introduced into the applicator (5mL syringe), and the No. 9 injection needle was used as the injection tube, connected to the positive electrode of the high-voltage power supply, and the aluminum foil microsphere receiving plate was connected to the negative electrode. The electrostatic spray process conditions are: voltage 10kv, flow rate 1.5mL / h, receiving plate distance 25cm, temperature 15°C, and air humidity 45%. The drug-loaded thermosensitive polymer microspheres were obtained. The observation picture of the polymer microspheres under the electron microscope is as follows: figure 1 shown.

[0031] Weigh 0.1g of phosphatidylcholine, dissolve it in 5ml of ethanol and chloroform (ethanol: chloroform = 1:1), add 0.5g of poly-N-isopropylacrylamide and 0.02g of ketoprofen, Stir at room temperature on a magnetic stirrer to obtain a temperature-sensitive polymer electrospray stock solution.

[0032] Introduce the prepared electrospray stock solution into the dosing device (5ml syringe), use a No. 9 injection needle as the injection tube, connect the positive electrode of the high-voltage power supply, and connect the aluminum foil microsphere receiving plate to the negative electrode. The electrostatic spray process conditions are: voltage 12kv, flow rate 1.0mL / h, receiving plate distance 20cm, temperature 25°C, and air humidity 80%. Acquired thermosensitive polymer microspheres. After vacuum drying, 2ml of high-purity water was added dropwise, evaporated to dryness naturally at 36°C, and observed with a transmission electron microscope, the results were as follows: f...

[0034] Weigh 0.2g of phosphatidylcholine, dissolve it in 10ml of ethanol and chloroform (ethanol: chloroform = 6:4), add 1g of poly-N-isopropylacrylamide and 0.08g of ketoprofen, and Stir on a stirrer at room temperature to obtain a temperature-sensitive polymer electrospray stock solution.

[0035] Introduce the prepared electrospray stock solution into the dosing device (5ml syringe), use a No. 9 injection needle as the injection tube, connect the positive electrode of the high-voltage power supply, and connect the aluminum foil microsphere receiving plate to the negative electrode. The electrostatic spray process conditions are: voltage 18kv, flow rate 0.5mL / h, receiving plate distance 10cm, temperature 20°C, and air humidity 57%. Acquired thermosensitive polymer microspheres. After vacuum drying, 10ml of high-purity water was added dropwise, evaporated to dryness naturally at 40°C, and observed with a transmission electron microscope, the results were as follows: image ...