Scylla paramamosain C-type lysozyme gene and application thereof

[0021] The present invention will be further elaborated below by following specific embodiments, but content of the present invention is not limited to this at all.

[0022] 1. Gene cloning of c-type lysozyme SpcLYZ from Scylla pseudosyrpa

[0023] 1.1 Total RNA extraction and cDNA synthesis

[0024] The blue crab (100g ± 10g) was purchased from Huangsha Market, Guangzhou, and its hepatopancreas, gills and muscles were mixed, and the total RNA was extracted with the marine animal RNA extraction kit of Tianenze Company, and the total RNA was measured with a UV spectrophotometer. Concentration and purity, and take 5 μL of total RNA and use 1.5% agarose to run the gel to ensure its integrity; use Takara’s reverse transcription kit to reverse-transcribe the qualified total RNA into cDNA, and store the synthesized cDNA in a -20°C refrigerator spare.

[0025] 1.2 SpcLYZ cDNA full-length clone

[0026] 1.2.1 cDNA fragment verification

[0027] According to the gene sequence in th...