18 results about "Pathogen" patented technology

The present invention provides methods for screening antimicrobial agents that make use of mutated microbial polypeptides. Because these polypeptides have biological activity and result in increased antimicrobial drug resistance, candidate compounds that specifically target these polypeptides provide therapeutics or therapeutic lead compounds for treating infections caused by drug resistant microbial pathogens.

The present invention provides kits and assay methods for the early detection of pathogens, precise identification of the etiologic agent, and improved disease surveillance. More specifically, the present invention discloses an immunoassay leading to the rapid and simultaneous detection of antibodies to a wide range of infectious pathogens in biological fluids of infected patients. This immunoassay involves the covalent and oriented coupling of fusion proteins comprising an AGT enzyme and a viral antigen on an identifiable solid support (e.g. fluorescent microspheres), said support being previously coated with an AGT substrate. This coupling is mediated by the irreversible reaction of the AGT enzyme on its substrate. The thus obtained antigen-coupled microspheres show enhanced capture of specific antibodies as compared to antigen-coupled microspheres produced by standard amine coupling procedures. The methods of the invention possess the ability to multiplex, minimize the amount of biological sample, and have enhanced sensitivity and specificity toward target antibodies as compared with classical ELISA or Radio-Immunoprecipitation assays.

The invention discloses a quantitative detection method for FOC (Fusarium oxysporum f. sp. cubense (E.F. Smith) Snyd. & Hans.) race 4 from the soil. The method comprises the following steps: pretreating a sample to be detected and extracting DNA of the sample to be detected; preparing specific primers; conducting fluorescent nucleic acid isothermal amplification detection technique RealAmp reaction; constructing a RealAmp standard curve; and determining the result. The real-time fluorescent nucleic acid isothermal amplification detection technology provided by the invention is a novel nucleic acid detection technology combining a new generation of isothermal nucleic acid amplification technology with the real-time fluorescent detection technology, and has the advantages of high sensitivity, high specificity, low pollution and stable reaction. RealAmp can quantitatively detect pathogens; because of the characteristics of LAMP, Bst DNA polymerase has chain exchange capacity and good tolerance of inhibiting substances contained in the soil DNA extracts; the method carries out detection work on the soil, avoids cover opening and influence by contaminants and conducts on-site soil sample detection; the method for analysis and determination of reaction products is very simple; therefore, the method is suitable for wide application.

The invention discloses acne-removing liquid containing a tea tree flower extract. The acne-removing liquid comprises the following components in percentage by weight: 20.0-50.0 percent of tea tree flower extract, 0.2-5.0 percent of plant essential oil, 0.5-5.0 percent of a natural plant source antibacterial agent, 0.2-2.0 percent of salicylic acid, 0.2-2.0 percent of vitamin E and the balance of water. The acne-removing liquid disclosed by the invention realizes the purposes of efficient and multi-effect treatment of acnes on the premise of taking natural plant ingredients without toxic and side effects as raw materials at the most extent: 1) inhibiting excessive secretion of sebum, regulating the oil balance of skin, converging oily and acne skin and improving skin immunity; 2) inhibiting the reproduction of propionibacterium acnes and controlling pathogens; 3) controlling inflammation, preventing the destruction of a hair follicle sebaceous gland structure and reducing the acnes; 4) desalting and removing the acnes, and improving the moisturizing effect of the skin; 5) adopting an antibacterial agent and a preservative serving as natural plants to reduce skin allergy symptoms.

The invention relates to a method for efficiently amplifying subgroup J avian leukosis virus (ALV). According to the method, chicken hepatocytes can be used for efficiently amplifying the subgroup J ALV, the characteristic of high titer virus can be produced earlier by using the chicken hepatocytes, the separation and detection time of the subgroup J ALV can be shortened, the pathogen can be detected earlier, and the sensitivity of a subgroup J ALV detection method is improved. When subgroup J ALV infection MOI is 0.001 hour, the effect of enzyme-linked immunosorbent assay (ELISA) on the chickhepatocytes infected for 3 days is comparable to the effect of ELISA on DF-1 cells infected for 7 days. The efficient amplification method established by the invention accelerates the purification process of the subgroup J ALV, thus shortening the time and reducing cost; the method has very good application value in the purification detection of the subgroup J ALV.

The present invention provides a safer way to intubate patients having communicable diseases, such as bacterial and viral infections. It protects health care professionals performing the intubation from bodily fluids of the patients that might otherwise be projected directly onto them by coughing, gagging, vomiting, or sneezing. Additionally, it traps airborne suspensions or aerosols containing pathogens, such as bacterial, myco-bacterial, viral, or fungal infections, originating from the patient and keeps them from entering into the air in close proximity to the healthcare professionals. The contaminated air can then be vented to a location which is away from personnel, such as an outdoor containment area. In one embodiment of this invention the contaminated air is passed through a disinfecting solution which destroys the pathogens before the air is recycled into the environment, such as the room where the intubation is carried out.

The present invention provides a rapid, highly sensitive and specific enzyme-linked immunosorbent assay (ELISA), referred to as N-Assay, a device, and a kit, for detection and identification of microorganisms in a sample, in thirty minutes or less, with little or no interference from non-target microorganisms. The present invention also provides for simultaneous determination of antimicrobial susceptibility of microorganism in the N-Assay.

The invention relates to multiple nested PCR (polymerase chain reaction) detection primer, kit and method for Neospora caninum, Brucella abortus and infectious bovine rhinotracheitis virus, and belongs to the technical field of molecular genetics. The multiple nested PCR detection kit comprises inner and outer primer pairs for detecting Neospora caninum ITS1, Brucella abortus 16S rRNA and infectious bovine rhinotracheitis virus gB gene; by using the kit, it is possible to synchronously detect 3 pathogens causing abortion of dairy cows. The multiple nested PCR detection method has very high sensitivity and specificity; infection conditions of 3 abortive pathogens can be detected at a time, the detection speed is high, and the cost is greatly saved. In the detection method, components of various PCR systems are optimized such that system composition is simple and reasonable; reaction procedures of each phase are optimized such that amplification results are easy to distinguish and amplification takes short time.

The invention relates to a durable antibacterial waterborne woodenware coating and a preparation method thereof. The coating comprises the following components: water soluble alkyd resin, a waterbornepolyurethane polyol dispersion body, an amino resin mixture, nano TiO2, nano ZnO, an association type thickener, a leveling agent, a defoamer, a basic wetting agent and deionized water, wherein the amino resin mixture is composed of methylated amino resin and ketone crosslinked polyamide polyamine epichlorohydrin with the weight ratio of 1:(0.2-0.6). The waterborne coating provided by the invention has durable antibacterial activity and mildew-proof performance, has a wide antibacterial spectrum, and has the killing effect on common pathogens such as staphylococcus aureus, streptococcus pneumonia, candida albicans, pseudomonas aeruginosa and aspergillus niger reaching 99.90% or more; through SGS ASTM F1980 tests, the woodenware coating still has the bacteriostasis rate of 99.30% or more 5years later, and has 5 years or more antibacterial effect.

The invention relates to a method for rapidly detecting a pathogen of the rice black-streaked dwarf. The method comprises the following steps: performing RT-PCR amplification by using a pair of virus specific primers of a rice black-streaked dwarf virus and a southern rice black-streaked dwarf virus and by taking the total RNA of a sample to be detected as a template, and analyzing a high resolution solubility curve of an amplification product under the sealing condition, thereby judging the pathogen of the rice black-streaked dwarf. The method has the benefits that aiming at the production practice, a method for rapidly and synchronously detecting and distinguishing RBSDV from SRBSDV with high flux by using only one pair of the primers without any other probe or step of electrophoresis after amplification, EB dyeing or observation in ultraviolet lamps is developed and established, and technical support is provided for accurate diagnosis, prediction and forecasting and scientific prevention and control on the rice black-streaked dwarf.

The invention discloses a nucleotide sequence opti-LTB-Asia 1/VP1 and a recombinant vector pC 1300/opti-LTB-Asia 1/VP1 containing the sequence. The invention further discloses fusion expression mucosal immune adjuvant Asia 1 type foot and mouth disease virus antigen prepared by using the recombinant vector pC 1300/opti-LTB-Asia 1/VP1 and an application thereof. The antigen expressed by transgenic tomatoes has good immunogenicity. The transgenic tomatoes only express sigmasubunit antigen, do not contain pathogenic microorganisms or potential pathogenic microorganisms and are safe for human and livestock and free of pathogen pollution. An extraction and purification process is not required by producing immunogen through transgenic plants, and the antigen, the preparation and the application have the advantages of being simple in process, good in immune effect and protective effect on animals and the like and are suitable for immunity of livestock.

The invention discloses a method for synchronously detecting five important virus pathogens in ranunculus asiaticus. The five important virus pathogens are ranunculus mild mosaic virus, ranunculus latent virus, ranunculus asiaticus white mottle virus, ranunculus asiaticus mosaic virus and ranunculus leaf distortion virus. According to the application of the method, plant total RNA or virus RNA isextracted, different types of virus cDNA first chains are synthesized by random primers, five viruses are subjected to multiplex PCR detection by specific primers, the steps of synthesizing the cDNA first chains by reverse transcription of a single virus primer, determining and analyzing a virus nucleotide sequence and the like are omitted, a target fragment is obtained by utilizing the rapidity and sensitivity of multiplex PCR, and a scientific method is established for detecting various ranunculus asiaticus virus disease samples quickly, accurately and sensitively in quantity.

The invention discloses a method for detecting infectious pathogens possibly existing in biological samples. The method comprises the following steps of: amplifying nucleic acid segments of the biological samples; detecting by using a dye reagent; and observing a detection result by naked eyes or detecting a detection result by means of ultraviolet light. The invention also provides a primer used for amplification and a complete set of device for detection. In the method, the result is directly observed by the naked eyes or by utilizing the ultraviolet light; and the detector has the advantages of simple operation, wide sample range and convenient carrying, detection on the first site where the epidemics happen, and suitability for early diagnosis of various pathogens including respiratory infectious disease.

The invention discloses a pesticide composition containing copper succinate and benziothiazolinone. The pesticide composition is prepared from effective active ingredients of copper succinate and benziothiazolinone, wherein the weight ratio of copper succinate to benziothiazolinone is (40 to 1) to (1 to 1). The pesticide composition provided by the invention has the advantages of excellent preventing and controlling effects on diseases such as downy mildew, anthracnose, bacterial angular leaf spot, canker and soft rot disease, long persistence period and safety for crops. The compound of copper succinate and benziothiazolinone has a remarkable synergistic effect; the use amount of the pesticide can be reduced, the use cost is reduced, the pollution to the environment is reduced, pesticideresistance of pathogen is delayed and the service life of the pesticide is prolonged.

The invention relates to a chicken Flavivirus and an inactivated vaccine thereof and belongs to the field of veterinary epidemiology. The chicken Flavivirus is chicken Flavivirus strain FQ-C1 which is preserved in the China General Microbiological Culture Collection Center with preservation serial number: CGMCC No.4905. After the chicken Flavivirus strain FQ-C1 is inoculated to SPF (specified pathogen free) chick embryos, the allantoic fluids of the SPF chick embryos which die of transfection are collected, the allantoic fluids of the SPF chick embryos are inactivated with formaldehyde and then mixed with white oil which is used as an adjuvant, and the resulting mixture is emulsified to prepare an inactivated vaccine in the form of oil emulsion. The chicken Flavivirus inactivated vaccine provided by the invention does not cause any adverse effect on chickens and has high experimental safety. The vaccine can effectively control Flavivirus disease in chickens and helps reduce the losses and increase the breeding benefits of majority of chicken farmers in China.