The invention discloses a method for measuring the
cobalt content of a
microorganism culture
system by an acidizing treatment method. The method is characterized by comprising the steps of putting 0.5-3.5 mL of a sample to be measured into a container with an opening, adding 65-68
mass percent of concentrated
nitric acid according to a volume ratio of 1:1, and oscillating and mixing the sample and the concentrated
nitric acid to be uniform, wherein the sample to be measured is a solution to be measured containing
cobalt ions in the
microorganism culture
system; heating the
mixed solution until the
mixed solution is boiling, oscillating to remove acid, evaporating the
mixed solution until the mixed solution is nearly dried, adding pure water for
smelting, losslessly transferring the mixture into a
volumetric flask with the volume of 10 mL, and finally adding water into the
volumetric flask to fix the volume to a scale line; measuring the
cobalt content of the
aqueous solution in the
volumetric flask by adopting a spectrophotometric method or / and an atomic absorption spectrometry. According to the method disclosed by the invention, the sample to be measured is subjected to acidizing treatment, so that the shortcoming that components of a
microorganism culture medium seriously affect cobalt measurement is overcome; meanwhile, other impurities cannot be introduced; therefore, the method disclosed by the invention has the advantages of simplicity, quickness, accuracy and sensitivity in operation and the like and can be successfully applied to the measurement of the cobalt content of the microorganism culture
system.