Method for measuring cobalt content of microorganism culture system by acidizing treatment method

[0052] Spectrophotometric determination of cobalt (100.00mg / L) in LB medium, the steps are as follows:

[0053] 1. Acidification:

[0054] Put 0.5-3.5mL sample into a 50mL small beaker, carefully add concentrated nitric acid at a volume of 1:1, and mix well after each addition; then put it on the electric furnace equipped with asbestos net until boiling, while shaking Rinse the acid until nearly dry, and add a small amount of pure water to dissolve it. After cooling to room temperature, transfer it to a 10mL volumetric flask without loss, dilute to volume with pure water, and mix well.

[0055] 2. Sample test:

[0056] 2.1 Spectrophotometric determination: draw 1.0mL of the above solution into a 25mL colorimetric tube, add 8.0mL ammonium citrate-ammonia buffer solution and 3.0mL picric azochromic acid respectively, dilute to the mark with pure water, shake Let it stand for 15 minutes evenly. Use a 1cm cuvette at a wavelength of 464nm to measure the absorbance with the reage...

[0084] Determination of cobalt (50.00mg / L) in LB medium, the steps are as follows:

[0085] The cobalt standard solution of 50.00 mg / L was configured with LB medium, and measured according to the steps in Example 1, the results are shown in Table 6:

[0086] Table 6: Determination results of cobalt ion (50.00mg / L) in LB medium:

[0087]

[0088] It can be seen from the above table 6 that the recovery rate of cobalt ion concentration after acidification of LB medium was determined from 80.10% and 75.20% to 96.00% and 98.56% by spectrophotometry and atomic absorption spectrometry.

[0090] Determination of cobalt (100.00mg / L) in NA medium

[0091] Configure 100.00 mg / L cobalt standard solution with NA medium, and measure according to the steps in Example 1, the results are shown in Table 7:

[0092] Table 7: Determination results of cobalt (100.00mg / L) in NA medium:

[0093]

[0094] It can be seen from Table 7 that after the LB medium is acidified according to the present invention, the cobalt ion concentration is measured by spectrophotometry and atomic absorption spectrometry at the same time, and the recoveries are increased from 83.77% and 88.17% to 98.01% and 98.17%, respectively.