Medicinal application of CREG protein in preventing or treating overweight, obesity and related diseases thereof

[0049] Example 1. Preparation of obesity model of C57BL / 6J mice fed with high fat and detection of CREG protein expression in adipose tissue.

[0050] ①Establishment of obesity model in C57BL / 6J mice.

[0051] Using random table method, 40 male 8-week-old C57BL / 6 mice [body weight (22.3±1.2) g] were divided into the following two groups: normal feeding group and high-fat feeding group, 20 mice in each group. The normal feeding group is fed with normal diet feed (3.85kcal / g, fat provides 10% of calories, U.S. Research Diet company), and the high-fat feeding group is fed with high-fat feed (5.24kcal / g, fat provides 60% of calories, U.S. Research Diet company). Continue feeding for 16 weeks. The mice were fed with standard feeding conditions, received 12 hours of light, and did not limit the amount of food and water they drank.

[0052] The results show that: after feeding for 16 weeks, observe the general shape of the mice, and find that the mice in the high-fat feeding group...

[0071] Example 2: CREG protein treatment significantly reduces obesity in high-fat fed C57BL / 6J mice.

[0072] ① CREG protein treatment can reduce obesity in mice induced by high-fat feeding.

[0073]Using the random table method, 60 male 8-week-old C57BL / 6 mice were divided into the following three groups: normal feeding group, high-fat feeding group, high-fat feeding + CREG protein treatment group. 20 in each group. Normal feeding and high-fat feeding methods are the same as in Example 1. In the high-fat feeding + CREG protein treatment group, at the same time as high-fat feeding, the mice were treated with CREG protein (Abcam, USA, 150 μg / kg·d) by subcutaneously embedding a micro-osmotic pump (Alzet, USA) for 16 week. The mice were fed with standard feeding conditions, received 12h of light, and did not limit the amount of food and water they drank. After 16 weeks, the general morphology of the mice was observed.

[0074] The results showed that after high-fat feeding,...

[0096] Example 3: CREG protein treatment significantly alleviated fatty degeneration in the liver of C57BL / 6J mice fed with high fat.

[0097] ①HE staining of the liver tissues of mice in the three groups.

[0098] When feeding for 16 weeks, the liver tissues of the mice in each group were taken for HE staining, and the specific method was the same as in Example 2.

[0099] The results showed that, compared with the normal-fed control group mice, the high-fat-fed mice had significant fatty degeneration in the liver, and the liver cells were occupied by a large number of lipid droplets. Compared with the mice in the high-fat-fed group alone, the degree of hepatocyte steatosis was significantly reduced after the mice in the high-fat-fed group were treated with CREG protein (for the results, see image 3 A), showing that CREG treatment antagonizes obesity-associated fatty liver in mice.

[0100] ②Comparison of liver tissue weight in three groups of mice.

[0101] After feeding...