Fixing method of high-activity glucose oxidase

[0036] Weigh 1.2g of HEMA, 0.4g of NVP, add 0.4% of photoinitiator Darocur 1173, 0.3% of cross-linking agent EGDMA into it, stir evenly to obtain a mixed solution; take 100 μL of the mixed solution, pour it into Add 10 mg of glucose oxidase, and sonicate to dissolve the enzyme completely. Take 7μL of the enzyme-dissolved solution and drop-coat it on the surface of the Pt electrode at a wavelength of 365nm and an intensity of 30mW / cm 2 Cured under ultraviolet light for 50 minutes, after curing, put the Pt electrode into PBS solution at room temperature for hydration, remove unreacted monomers, and obtain glucose oxidase-modified electrodes.

[0038] Weigh 1g of HPMA, 0.6g of NVP, add 0.4% photoinitiator Darocur 1173, 0.3% cross-linking agent EGDMA to it, stir well to obtain a mixed solution; take 100 μL of the mixed solution, add 10mg of glucose oxidase, ultrasonic treatment to dissolve the enzyme completely. Take 7μL of the enzyme-dissolved solution and drop-coat it on the surface of the Pt electrode at a wavelength of 365nm and an intensity of 30mW / cm 2 Cured under ultraviolet light for 50 minutes, after curing, put the Pt electrode into PBS solution at room temperature for hydration, remove unreacted monomers, and obtain glucose oxidase-modified electrodes.

[0039] figure 2 The current response to glucose of the sensor prepared by using HEMA and NVP as the photosensitive resin curing enzyme of the hydrophilic monomer in Example 1 of the present invention; image 3 The current response of the sensor made for the photosensitive resin solidifying enzyme of the embodiment 2 of the present invention using HPMA an...

[0041] Weigh 0.6g of HPMA, 1g of NVP, 0.4g of VTEO, add 0.4% of photoinitiator Darocur1173, 0.3% of cross-linking agent EGDMA, stir well to obtain a mixed solution; take 100 μL of mixed solution , adding 10 mg of glucose oxidase to it, and ultrasonically dissolving the enzyme. Take 7μL of the enzyme-dissolved solution and drop-coat it on the surface of the Pt electrode at a wavelength of 365nm and an intensity of 30mW / cm 2 Cured under ultraviolet light for 50 minutes, after curing, put the Pt electrode into PBS solution at room temperature for hydration, remove unreacted monomers, and obtain glucose oxidase-modified electrodes.