Ganoderma sinense mycelium polysaccharide refined substance as well as preparation method and use thereof
一种菌丝体多糖、紫芝的技术,应用在药物组合、医药配方、含有效成分的医用配制品等方向,能够解决未涉及多糖分子多糖单糖等问题,达到明显抗肿瘤活性、显著抗肿瘤作用、给药量小的效果
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Embodiment 1
[0060] Crude polysaccharide extraction:
[0061] Ganoderma sinense was used for liquid fermentation to obtain 1300 L of fermentation broth, the obtained mycelium was washed with water, and plate-and-frame filter press was performed. The mycelium was extracted twice with 3 times of water and heated for 3 hours each time, and the extraction temperature was 90°C. The water extracts were combined and concentrated until the ratio of the amount of mycelium to the amount of medicinal solution was 1:2, and then 4 times of 95% alcohol was added to precipitate overnight, and the precipitate was taken out and dried to obtain 119.22 g of crude polysaccharide.
[0062] The polysaccharide content of the crude polysaccharide measured by the phenol sulfuric acid method was 34.9%.
[0063] Crude polysaccharide refining:
[0064] Take the above crude polysaccharide and dissolve it in 20 times of water, separate the crude polysaccharide solution with an ultrafiltration membrane with a molecula...
Embodiment 2
[0068] Crude polysaccharide extraction:
[0069] Ganoderma sinense was used for liquid fermentation to obtain 1300 L of fermentation broth, the obtained mycelium was washed with water, and plate-and-frame filter press was performed. The mycelium was extracted with 3 times of water and heated for 3 times, each time for 2 hours, and the extraction temperature was 80°C. The water extracts were combined and concentrated until the ratio of the amount of mycelium to the amount of medicinal solution was 2:1, and then added 3 times of 85% alcohol to precipitate overnight, and the precipitate was taken out and dried to obtain 144.46 g of crude polysaccharide.
[0070] The polysaccharide content of the crude polysaccharide measured by the phenol sulfuric acid method was 42.8%.
[0071] Crude polysaccharide refining:
[0072] Take the above crude polysaccharide and dissolve it in 20 times of water, separate the crude polysaccharide solution with an ultrafiltration membrane with a molec...
Embodiment 3
[0076] Crude polysaccharide extraction:
[0077] Ganoderma sinense was used for liquid fermentation to obtain 1300 L of fermentation broth, the obtained mycelium was washed with water, and plate-and-frame filter press was performed. The mycelium was extracted with 3 times of water and heated for 3 times, each time for 2 hours, and the extraction temperature was 80°C. The water extracts were combined and concentrated until the ratio of the amount of mycelium to the amount of medicinal solution was 2:1, and then added 3 times of 85% alcohol to precipitate overnight, and the precipitate was taken out and dried to obtain 144.46 g of crude polysaccharide.
[0078] The polysaccharide content of the crude polysaccharide measured by the phenol sulfuric acid method was 42%.
[0079] Crude polysaccharide refining:
[0080] The above crude polysaccharide was dissolved in 20 times of water, the crude polysaccharide liquid was separated by an ultrafiltration membrane with a molecular wei...
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