10 results about "Solid phase extraction" patented technology

The technology of the present application is directed to methods and kits for detecting and quantifying a non-polar analyte in a plant-derived sample. The technology uses a simple extraction, e.g., a liquid-liquid extraction (LLE) or solid-phase extraction (SPE), to enrich a sample for a non-polar analyte of interest and to remove contaminants. After the extraction and clean-up steps, liquid chromatography and mass spectrometry are used to detect the non-polar analyte. In one embodiment, acequinocyl and/or its derivatives is analyzed using liquid chromatography and tandem mass spectrometry (LC-MS/MS) and the improved LC-MS/MS conditions allows detection limits of acequinocyl and/or its derivatives of 50 ppb or less.

The invention discloses a mold for preparing a solid-phase extraction stirring bar and a preparation method thereof. The mold comprises a coating layer molded member and a pedestal; the central part of the coating layer molded member is provided with a circular through hole; the pedestal is a disk having the central part provided with a molded member positioning groove and a stirring bar positioning groove. The preparation method comprises the following steps: the stirring bar is inserted into the stirring bar positioning groove of the mold; then a film-casting liquid is injected into the mold, and the film-casting liquid is subjected to in situ solidification molding in the mold through chemical reaction, heating and illumination ways; and the stirring bar and an extraction coating layer coating the exterior of the stirring bar are together divorced from the mold. The method for preparing the solid-phase extraction stirring bar by utilizing the mold molding way has the characteristics of no need of large equipment, simple and fast manufacturing method, good controllability of the extraction coating layer size, high combination degree of the extraction coating layer with the stirring bar and the like.

The invention discloses a processing method for enhancing aroma of fruit baijiu. The processing method comprises the following steps: carrying out synergistic treatment on fruit dry wine by adopting ultrasonic waves and ultraviolet rays, thus obtaining treated liquid; carrying out headspace solid-phase extraction on the obtained treated liquid, thus obtaining aroma substances, carrying out cooling, and carrying out sealed storage; carrying out pulsed electric field treatment on the raffinate after extraction, and carrying out distillation, thus obtaining base wine; and finally, adding the stored aroma substances into the obtained base wine, and carrying out low-temperature ageing, thus obtaining the fruit baijiu. The fruit baijiu obtained by adopting the processing method is rich in fruity aroma, rich in esters, refreshing ad smooth in mouthfeel, and low in content of fusel oil, and the effects of alcohol can not move to the head after a person drinks much fruit baijiu. The processing method has the advantages that the process is simple, and the cost is low.

The invention belongs to the field of medical examination, and relates to an in vivo antibacterial drug content determination method, in particular to an online solid phase extraction liquid chromatography for detecting content of linezolid in blood. The online solid phase extraction liquid chromatography for detecting the content of linezolid in the blood performs pretreatment on a to-be-detectedblood sample and then determines the content of linezolid by virtue of a high performance liquid chromatograph and by adopting a standard curve method. The online solid phase extraction liquid chromatography for detecting linezolid drug concentration in the blood has the advantages that blood plasma is subjected to protein precipitation and then is directly injected, operation of personnel is reduced and accuracy of a quantitative result is improved.

The invention discloses a method for detecting alkaline colorants in livestock meat products. The method for detecting the alkaline colorants in the livestock meat products comprises the steps that extraction of samples to be detected is carried out by using an extraction solution, a liquid supernatant obtained after extraction is purified by a WCX solid-phase extraction column to complete samplepretreatment, and then, the detection method for simultaneously screening and confirming the seven alkaline colorants in the livestock meat products is established by using an ultra-high efficient liquid chromatography-series connection quadrupole rod linear ion trap mass spectrometer. The method for detecting the alkaline colorants in the livestock meat products has the characteristics of simpleoperation, efficient flux, qualitative accuracy, high sensitivity, friendly environment and the like, is suitable for screening and confirming the various alkaline colorants in the meat products, andavoids detection of false positive samples.

The present invention is hygroplasm combining detection method for detecting several kinds of drugs in urine. The present invention features that based on practical sample condition and required detection sensitivity, the sample is first pre-treated via solid phase extraction, direct deposition or direct sample feeding, and then gradient eluted in linked chromatographic column with SKF525A as internal reference and water or methanol containing formic acid as flow phase. The method is simple and reliable in the quantitative detection of 19 kinds of drugs and has its methodologic indexes meeting the practical requirement.