Preparation method of ginsenoside Rg1

A technology of ginsenosides and total saponins, applied in the directions of steroids, organic chemistry, etc., can solve the problems of high cost and difficult industrialized production, etc.

Inactive Publication Date: 2009-12-09
PHARMA RES INST OF BENCAO TIANYUAN OF BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The application number is the patent document of 03148804.8, and this document discloses the method for adopting silica gel (or aluminum oxide) column chromatography to obtain ginsenoside Rg 1 ; The application number is the patent literature of 200610093615.9, 200610093610.6, which discloses that the method for adopting silica gel column chromatography, alumina column chromatography or ODS column chromatography can obtain ginsenoside Rg 1 ; This type of method is expensive, only applicable to laboratory research, and is difficult to realize industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] The Panax notoginseng total saponins obtained by the extraction of Panax notoginseng were dissolved in water, filtered, the filtrate passed through the HPD-400A macroporous adsorption resin column, washed with water, the eluent was discarded, eluted with 20% ethanol, the eluate was collected, concentrated, and dried. Dissolve the dry matter in water, filter, and pass the filtrate through the HPD-400A macroporous adsorption resin column, wash with water, discard the eluent, then elute with 10% ethanol, discard the eluent, elute with 20% ethanol again, collect The eluate was concentrated and dried to obtain ginsenoside Rg 1 The crude product is extracted by adding organic solvent acetone, the extract is filtered, the filtrate recovers the solvent to dryness, and the residue is dried to obtain ginsenoside Rg 1 .

[0069] The method for extracting total saponins from Panax notoginseng is prepared according to existing patent documents or scientific documents.

[0070] The...

Embodiment 2

[0072] Take the total saponins extracted from ginseng and dissolve in water, filter, pass the filtrate through HPD-400 macroporous adsorption resin column, wash with water, discard the eluent, elute with 60% ethanol, collect the eluate, concentrate, dry, add water to the dry matter Dissolve, filter, pass the filtrate through HPD-400 macroporous adsorption resin column, wash with water, discard the eluent, then elute with 50% ethanol, discard the eluent, then elute with 60% ethanol, collect the eluate , concentrated and dried to obtain ginsenoside Rg 1 The crude product is extracted by adding organic solvent butanone, the extract is filtered, the filtrate recovers the solvent to dryness, and the residue is dried to obtain ginsenoside Rg 1 .

[0073] The extraction method of total ginseng saponins is prepared according to existing patent documents or scientific documents.

[0074] The above-mentioned ginsenoside Rg 1 As pharmaceutical raw materials, it can be prepared into ph...

Embodiment 3

[0076] Extract the total saponins obtained from American ginseng, dissolve them in water, filter, pass the filtrate through a HPD-300 macroporous adsorption resin column, wash with water, discard the eluent, elute with 25% ethanol, collect the eluate, concentrate, dry, and dissolve the dry matter in water , filtered, the filtrate was passed through a HPD-300 macroporous adsorption resin column, washed with water, and the eluent was discarded, then eluted with 15% ethanol, the eluent was discarded, and then eluted with 25% ethanol, and the eluate was collected. Concentrate and dry to get ginsenoside Rg 1 The crude product is extracted by adding organic solvents n-butanol, ethanol, and methanol, the extract is filtered, the filtrate is recovered from the solvent to dryness, and the residue is dried to obtain ginsenoside Rg 1 .

[0077] The extraction method of total saponins of American ginseng is prepared according to existing patent documents or scientific documents.

[0078...

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PUM

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Abstract

The invention discloses a preparation method of active principles of ginsenoside Rg1, which is characterized in that total saponins are obtained from different drug ingredients, and the ginsenoside Rg1 with the purity being larger than 90 percent is obtained by a method of organically combining a macroporous absorbent resin method and organic solvent extraction. The preparation method is suitable for industrialized production, can obtain the ginsenoside Rg1 in kilogram and has high extraction yield. Pharmacological tests show that the ginsenoside Rg1 obtained by the preparation method has good drug action.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine, in particular to a kind of ginsenoside Rg 1 The method of preparation of the active ingredient. Background technique [0002] Ginsenoside Rg 1 It is mainly derived from the roots, stems, leaves, and flower buds of plants such as Araliaceae Panax ginseng, Panax notoginseng, Siyang ginseng, Vietnamese ginseng, bamboo joints, Cucurbitaceae Gynostemma genus Gynostemma, Yuanguo Gynostemma, etc. It has good pharmacological properties Activity: (1) Protective effect on myocardial ischemia-reperfusion, studies have shown that ginsenoside Rg 1 It can reduce the injury of myocardial ischemic cells in SD neonatal mice, and exert a protective effect on myocardial ischemia-reperfusion; (2) the protective effect on cerebral ischemia, ginsenoside Rg 1 Can increase cerebral blood flow by improving animal microcirculation, play the effect of improving cerebral ischemia; (3) have antithromb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J17/00
Inventor 顾群李志刚郭小鹏米长江阮爱华刘严渠守峰金治刚林治荣
Owner PHARMA RES INST OF BENCAO TIANYUAN OF BEIJING
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