Application of GOLT1B inhibitor in preparation of medicine for treating colorectal cancer

[0025] Example 1 GOLT1B is significantly highly expressed in colorectal cancer

[0026] Using the Oncomine online database to analyze the mRNA data related to colorectal cancer, it was found that GOLT1B was significantly highly expressed in colorectal cancer ( figure 1 , figure 2 ); CPTAC (Clinical Proteomic Tumor Analysis Consortium, Clinical Proteomic Tumor Analysis Collaborative Group) data showed that the protein expression level of GOLT1B in colorectal cancer patients was significantly higher than that in the normal group ( image 3 ); immunohistochemical analysis was consistent with the results of the database ( Figure 4 ); while patients with high expression of GOLT1B had a lower survival rate ( Figure 5 ).

[0027] The cultivation of embodiment 2 cells

[0028] Colorectal cancer cell lines HCT116 and RKO were preserved by the Institute of Gastroenterology, Sun Yat-Sen University. HCT116 used McCoy's 5a Medium Modified (Gibco, USA) medium containing 10% fetal calf serum (Invitrogen, USA), and RKO used 10% fetal bovine serum (Invitrogen, USA) containing 10% fetal bovine serum (Invitrogen, USA). The MEM (Gibco, USA) medium of bovine serum (Invitrogen, USA) was cultured in a 37° C. constant-temperature humid incubator containing 5% carbon dioxide.

[0029] Embodiment 3 inhibitor suppresses GOLT1B gene expression

[0030] The HCT116 and RKO cells cultured in Example 2 were inoculated into a six-well plate containing an appropriate amount of complete medium, and the inoculation amount was 1×10 6 cells, so that the cell density during transfection can reach 30%-50%, use the siRNA of GOLT1B (the sequence of siRNA is shown in Table 1) and the control group si-NC to transiently transfect HCT116 and RKO cells, siRNA and si-NC The final concentration of NC was 5OnM. Replace with fresh complete medium without antibiotics 12 hours after transfection.

[0031] After the transfected cells were cultured for 48 hours, the total RNA was extracted to detect the expression level of the target GOLT1B by RT-PCR. The results were as follows: Image 6 As shown, the cells showing the transfection inhibitor (treated group) had significantly lower expression levels of the target than the control group.

[0032] Table 1 Sequence information of...