Dried blood spot quality control product for neonatal screening and preparation method thereof

[0063] S2. Preparation of blank filter paper

[0064] Place the blank filter paper in an oven at 65°C for 1.5 hours;

[0065] S3, preparation of dried blood spot quality control

[0066] Take 100 μL of the mixed standard containing 37 standard substances and add it to 900 μL of blood, mix well to make blood working solution. Use a pipette gun to pipette 50 μL of blood working solution, slowly add it to the filter paper ring, and after the blood is initially coagulated, move it to a clean room to dry naturally for 6 hours. The air-dried blood spot filter paper is the dried blood spot quality control product, which is stored in a -20°C refrigerator (to avoid organic pollution).

[0067] S4. Judgment of qualified blood spot: the blood sample completely and naturally infiltrates in the circle of the blood spot, and the diameter of the formed blood spot is 10-12mm.

[0068] The high and low concentrations of the dried blood spot quality control substances involved are shown in T...

[0073] Embodiment 1, the way of spotting blood on dried blood spot filter paper is determined

[0074] Mix blood and water at a ratio of 9:1, draw a certain amount of blood mixture with a calibrated 100 μL pipette gun, control the distance between the tip and the center of the blood spot, and slowly add blood to the Whatman 903 filter paper dry blood spot collection card (printed The diameter of the circle is 10mm), check the diffusion of blood in the filter paper, and judge whether the blood spot is qualified or not by visual inspection.

[0075] Embodiment 2, determination of spot blood volume

[0076] In Example 1, 10 μL, 25 μL, 50 μL, and 75 μL of blood samples were respectively added to the dry blood spots on filter paper, and the diffusion of different blood addition amounts was observed. From the point of view of the formation of blood spots, the blood spots formed when the spot blood volume was 50 μL just covered the diameter of the impression circle (10 mm).