6 results about "Petri dish" patented technology

The invention discloses a ceramic tile anti-mite effect testing method which comprises the following steps: S1, placing a to-be-tested ceramic tile and a contrast ceramic tile in a culture dish in a side-by-side tight butt joint manner, respectively arranging mite feed areas on the surfaces of the butt joint parts of the two ceramic tiles, photographing the feed areas under an ultraviolet lamp, and respectively calculating the areas of the feed areas on the two ceramic tiles, the feed in the mite feed area is mixed with a fluorescent compound which emits fluorescence under the irradiation of ultraviolet light; s2, acarid is put into the center of the feed area, and the culture dish is put under the proper condition for culture; s3, after culturing for a certain period of time, putting theculture dish under an ultraviolet lamp, photographing, and respectively calculating areas of residual feed areas on the two ceramic tiles, and S4, calculating according to a formula shown in the specification so as to obtain an anti-mite index K; the method can realize rapid and programmed detection of the anti-mite effect of the ceramic tile, and has the characteristics of easy operation, easy result statistics and good evaluation result repeatability.

The invention provides a preparation method of mouse bone marrow macrophages. The preparation method comprises the steps as follows: (1) preparing a complete medium; (2) dislocating cervical vertebraof mice of 6-9 weeks old; (3) taking femur and tibia of the mice, blowing out mouse bone marrow cells, repeating the operation until the bone is colorless, collecting a single cell suspension after blowing, performing centrifuging, and discarding a supernatant; (4) adding a red blood cell lysis buffer to cell precipitate for treatment; (5) adding the mixed solution to a complete medium rapidly forneutralization, performing centrifuging, and discarding a supernatant; (6) adding the complete medium to centrifugal cell precipitate to resuspend cells; (7) inoculating a petri dish with the resuspended cells for culture; (8) after 4 hours, sucking out the suspension cells, inoculating a petri dish of the complete medium containing an inducer with the cells, and recording the day as the first day; (9) changing half of the medium of the cells on the third day, changing the medium every other day until the seventh day, and performing testing. A simple, economical and rapid method for separating the mouse bone marrow macrophages is provided.

The invention discloses an immobilization method and application of feather degrading bacteria. Comprising the following steps: inoculating a feather degrading bacterium, namely Chryseobacterium, into an LB liquid culture medium to obtain fermentation liquor; waste biomass is taken as a carrier, is crushed and sterilized, and is subjected to oscillation adsorption with the bacterial liquid; after the adsorption is finished, putting the immobilized feather degrading bacteria into a culture dish, adding tetramethyl orthosilicate for sealed reaction to obtain immobilized feather degrading bacteria, and adding the immobilized feather degrading bacteria into a feather fermentation liquid to degrade feathers and obtain an amino acid fermentation liquid. According to the microorganism immobilization method disclosed by the invention, the waste biomass is used as a carrier, so that the microorganism is prevented from being in contact with a silicon precursor and a by-product in a silica sol-gel process, a required carbon source is also provided for a bacterial strain immobilized on the microorganism, and the microorganism immobilization method has good biocompatibility. And the silica gel process can prevent the leakage of the microorganisms adsorbing the immobilized microbial agent and enhance the stability of the microorganisms. The finally prepared immobilized microbial agent is high in feather degradation rate, easy to recycle and capable of being used for multiple times.

The invention relates to a method for improving the germination rate of forage grass seeds under salt stress, in particular to a method for improving the germination rate of the forage grass seeds under salt stress by treating the forage grass seeds with a calcium propionate solution having a concentration of 0.1-1.0%. The method comprises the following steps: adding a neutral salt solution and a calcium propionate solution into a culture dish paved with double-layer filter paper, putting leguminous grass seeds having been subjected to surface disinfection into the culture dish, and carrying out a germination test in an illumination incubator with a constant temperature of 25 DEG C. The method for improving the germination rate of the forage grass seeds under the salt stress can greatly improve the germination rate of the forage grass seeds under the salt stress condition, is mildew-proof and bacteriostatic, can prevent the occurrence of forage grass diseases to a certain extent, and is safe, effective, green and environment-friendly.

The invention discloses a simple and convenient identification method of bacillus cereus for konjac glucomannan, which comprises the following steps: step 1, sample preparation: weighing a konjac glucomannan raw material sample under a sterile condition, adding normal saline, and stirring for dissolving; step 2, pre-screening targeted bacteria: putting the solution prepared in the step 1 into a constant-temperature furnace for heating and pre-cooking, and after the temperature is raised to 88-92 DEG C, carrying out heat preservation and pre-screening to obtain spore bacteria; step 3, sterile inoculation: sucking the solution pretreated in the step 2 into a sterile culture dish, adding a compound culture medium solution, and rotating the culture dish to uniformly mix; step 4, culturing: after the agar is solidified, turning over the plates, and culturing at 36 +/-1 DEG C for 48 +/-2 hours; and step 5, identifying, wherein cultured test colonies show colonies in the shape of snowflake or plum blossom. By means of the method, the raw materials containing the bacillus cereus and possibly having risks can be screened out in time within the shortest time, and the safety of jelly food is improved.

The invention discloses a CHA gelatin stent which is prepared by the following steps: dissolving 5g of B-type gelatin from bovine bone into 50ml of distilled water containing sodium citrate dehydrate to serve as a dispersing agent; after the gelatin is completely swelled, adding 0.37 g of calcium hydroxide, and mixing for 30 minutes by using a magnetic stirrer at 37 DEG C at the rotating speed of 200 rpm; diluting 0.24 g of phosphoric acid in 50 ml of distilled water, and titrating the obtained phosphoric acid solution into the gelatin hydrogel solution for 15 minutes; continuing the synthesis maturity for 3 hours, regularly controlling the pH, and finally regulating the pH to 7.4; weighing 9.5 g of a Gel-CHA05 solution and pouring into a solution culture dish of 8 x 8 cm < 2 >, drying the solution culture dish in a refrigerator of 4 DEG C and then placing in a vacuum oven to be heated at 142 DEG C for 72 h so as to induce cross-linking formation of the stent, and performing gas sterilization in low-temperature ethylene oxide.