Method for rapidly excavating and measuring adenosylmethionine in Baijiu making process

[0033] Determination method of adenosylmethionine content in fermented grains in the production of Niulanshan Baijiu.

[0034] Weigh 10g of wine fermented grains containing adenosylmethionine after genomic and transcriptome analysis into a 50mL Erlenmeyer flask, add 20ml of ultrapure water, soak for 30min, ultrasonically extract 3 times, each time for 20min, centrifuge at 12000rpm for 15min and take The supernatant is ready for use; add 2ml of methanol to the oasis HLB extraction column to activate the solid phase extraction column, and then add 1ml of ultrapure water to balance the extraction column; add the supernatant obtained by the above centrifugation, let stand for 10min, add 2ml of methanol to elute, and then add 1ml of mobile phase (water (containing 0.1% formic acid):methanol=(95:5, v / v)) to elute, and collect 0.8ml of effluent; pass through a 0.22μm filter membrane and put it in a liquid phase vial for LC-MS detection Standby; follow the LC- MS analysis conditions ...

[0036] Determination method of adenosylmethionine content in distiller's grains in the production of Niulanshan liquor.

[0037] Weigh 8g of distiller's grains containing adenosylmethionine after genomic and transcriptome analysis into a 50mL Erlenmeyer flask, add 18ml of ultrapure water, soak for 20min, ultrasonically extract twice, each time for 25min, centrifuge at 12,000rpm for 13min and take out The supernatant is ready for use; add 2ml of methanol to the oasis HLB extraction column to activate the solid phase extraction column, and then add 1ml of ultrapure water to balance the extraction column; add the supernatant obtained by the above centrifugation, let it stand for 10min, add 2ml of methanol to elute, and then add 1ml Elute with mobile phase (water (containing 0.1% formic acid):methanol=(95:5, v / v)), and collect 0.8ml of the effluent; pass through a 0.22μm filter membrane and put it in a liquid phase vial for LC-MS detection for later use ; According to (column temp...

[0039] Determination method of adenosylmethionine content in Daqu in the production of Niulanshan Baijiu.

[0040] Weigh 5g of Daqu containing adenosylmethionine after genome combined with transcriptome analysis into a 50mL Erlenmeyer flask, add 15ml of ultrapure water, soak for 10min, ultrasonically extract twice, each time for 20min, centrifuge at 12,000rpm for 10min and take out The supernatant is ready for use; add 2ml of methanol to the oasis HLB extraction column to activate the solid phase extraction column, and then add 1ml of ultrapure water to balance the extraction column; add the supernatant obtained by the above centrifugation, let it stand for 10min, add 2ml of methanol to elute, and then add 1ml Elute with mobile phase (water (containing 0.1% formic acid):methanol=(95:5, v / v)), and collect 0.8ml of the effluent; pass through a 0.22μm filter membrane and put it in a liquid phase vial for LC-MS detection for later use ; According to (column temperature: 25°C; mobi...