6 results about "Salmonella" patented technology

The invention relates to a method for fast detecting pathogenicbacteria in food, in particular to a loop-mediated isothermal amplification method for detecting salmonella in food by using HisJ as target genes and a reagent kit. The detecting method uses saimonella HisJ genes as the target genes, four loop-mediated isothermal amplification specific primers are used for detecting the salmonella, and the sequences of the four loop-mediated isothermal amplification specific primers are shown as SEQ ID NO 1-4. The method has the advantages that the operation is simple, the detection time is short,the specificity is high, the sensitivity is high, the application range is wide, and the like, and the traditional method for detecting the salmonella in the food can be replaced.

The invention discloses PCR (polymerase chain reaction) detection primers for three bacteria and the application of the PCR detection primers. The three bacteria are alicyclobacillus aacillus, escherichia coli and salmonella. The upstream detection primer of the alicyclobacillus aacillus has the SEQIDNO.1, and the downstream detection primer has the SEQIDNO. 2. The upstream detection primer of the escherichia coli has the SEQIDNO.3, and the downstream detection primer has the SEQIDNO.4. The upstream detection primer of the salmonella has the SEQIDNO.5, and the downstream detection primer has the SEQIDNO.6. The multiple PCR detection method provided by the invention has the biggest advantages that the one PCR tube can be used for detecting three bacteria, the PCR detection efficiency is improved, and the errors caused by misoperation during detection are reduced.

The invention discloses a novel coronavirus (SARS-CoV-2) vaccine antigen presentation system of attenuated salmonella secretory expression NTD structural domain protein and application of the novel coronavirus (SARS-CoV-2) vaccine antigen presentation system. The novel coronavirus (SARS-CoV-2) vaccine antigen presentation system is used for preventing novel coronavirus (SARS-CoV-2). By constructing the controllable and stable expression plasmid for secreting and expressing the NTD structural domain protein and performing engineering modification on the attenuated salmonella, the antigenic protein can be efficiently delivered to an antigen presenting cell by virtue of a specific secretion system of the attenuated salmonella after administration in an oral way. The delivered antigenic protein can be effectively treated and presented by antigen presenting cells, and finally, the immune system is activated/regulated, an antibody is generated, and the effect of a vaccine is achieved.

Organic compounds showing the ability to inhibit effector toxin secretion or translocation mediated by bacterial type III secretion systems are disclosed. The disclosed type III secretion system inhibitor compounds are useful for combating infections by Gram-negative bacteria such as Salmonella spp., Shigella flexneri, Psendomonas spp., Yersinia spp., en tero pathogenic and enteroinvasive Escherichia coli, and Chlamydia spp. having such type III secretion systems.