Analogous CecroPinP1 antimicrobial peptide and application thereof

[0018] The solid-phase chemical synthesis method of embodiment 1 class CecroPinP1 antimicrobial peptide

[0019] The amino acid sequence of the CecroPinP1-like in this example is: Lys Asn Ser Lys Arg Lys Glu GlyLys Ser Glu Gly Ile Ala Ile Ala Ile Gln Gly Lys Arg (same as shown in SEQ ID NO: 1), and its molecular weight is 2298.65u. The CecroPinP1-like structure includes the Ser Glu Gly Ile Ala Ile Ala IleGln Gly antibacterial active structure of CecroPinP1, and the flexible and curved helical coil structure formed by Glu-Gly; at the same time, it is rich in Lys residues, which can attract negative charges on the surface of bacteria.

[0020] According to the amino acid sequence of the CecroPinP1-like antimicrobial peptide, the CecroPinP1-like antimicrobial peptide was artificially synthesized according to a standard solid-phase peptide synthesis procedure. The synthesized peptide was desalted and purified by reverse phase-high pressure liquid chromatography (RP-HPLC, Column: X...

[0021] The preparation of embodiment 2 class CecroPinP1 antimicrobial peptide tablet

[0022] The application of the CecroPinP1-like antibacterial peptide in the preparation of antibacterial drugs in this example, the amino acid sequence of the antibacterial peptide is: Lys Asn Ser Lys Arg Lys Glu Gly Lys Ser Glu Gly Ile Ala Ile Ala Ile GlnGly Lys Arg, the molecular weight is 2298.65u . The CecroPinP1-like antimicrobial peptide is made into CecroPinP1-like polypeptide tablet by conventional method. The content of each tablet of CecroPinP1-like antibacterial peptide is 0.2g, the percentage of CecroPinP1-like antibacterial peptide is 40%, and the auxiliary materials are starch, brown sugar and talc. pink.

[0023] Example 3 Preparation of CecroPinP1 Antimicrobial Peptide Sodium Chloride Injection

[0024] In this embodiment, the application of the CecroPinP1-like antibacterial peptide in the preparation of antibacterial drugs, the amino acid sequence of the antibacterial peptide is: Lys Asn Ser Lys Arg Lys Glu Gly Lys Ser Glu Gly Ile Ala Ile Ala IleGln Gly Lys Arg, the molecular weight is 2298.65 u. The CecroPinP1-like antimicrobial peptide is made into the CecroPinP1-like polypeptide bacteriocin sodium chloride injection by a conventional method, and each 100mL injection is composed of the following components: 0.5g of the main drug-like CecroPinP1 antimicrobial peptide, and the percentage content of the CecroPinP1-like antimicrobial peptide is 0.5%. , sodium chloride 0.9g, and the balance is distilled water for injection.