6 results about "Sample preparation" patented technology

The invention belongs to the field of molecular biological detection and identification and provides a rapid microbiological inspection and quarantine method for entry and exit ports. The method mainly includes steps of sample collection, sample preparation, high-throughput sequencing, bioinformatics analysis and association analysis. The method has advantages that on the basis of metagenomics, qualitative and quantitative detection of microbial community structures at the entry and exit ports can be realized, and high sensitivity and accuracy of results are achieved.

The invention discloses a blank oil sample preparation device and method for transformer insulating oil. The device is characterized in that the device comprises a clean displacement chamber for containing the insulating oil, a gas input assembly for inputting inert gas into the clean displacement chamber, a gas exhaust assembly which is introduced into the clean displacement chamber and is used for exhausting, and an oil sample output assembly which is connected with the clean displacement chamber and is used for sampling and oil treatment; and the gas input assembly inputs gas into the cleaning chamber to perform bubbling purging on the insulating oil, and presses the oil sample into the oil sample output assembly after bubbling purging. According to the invention, the clean displacementdegree of dissolved gas in the insulating oil is effectively controlled, the preparation reliability of the blank oil sample is improved, the air pressure of the clean displacement chamber is stablein the preparation process, burst and oil splashing are avoided, the safety in the preparation process of the blank oil sample is improved, and the high-quality blank oil sample with stable clean displacement degree can be obtained; and the sampling process is simple to operate, safe and free of pollution.

The invention discloses a detection method for diglycolamine residues The detection detection method comprises the following steps of: extracting diglycolamine in a to-be-detected sample by using an ultrasonic extraction method, and detecting the diglycolamine residues by using a gas chromatograph. The detection method of the diglycolamine residues is simple in operation, convenient in sample preparation, sensitive in response and good in separation degree, and can be used for rapidly and accurately detecting the diglycolamine residues in the polysaccharide.

The invention discloses a simple and convenient identification method of bacillus cereus for konjac glucomannan, which comprises the following steps: step 1, sample preparation: weighing a konjac glucomannan raw material sample under a sterile condition, adding normal saline, and stirring for dissolving; step 2, pre-screening targeted bacteria: putting the solution prepared in the step 1 into a constant-temperature furnace for heating and pre-cooking, and after the temperature is raised to 88-92 DEG C, carrying out heat preservation and pre-screening to obtain spore bacteria; step 3, sterile inoculation: sucking the solution pretreated in the step 2 into a sterile culture dish, adding a compound culture medium solution, and rotating the culture dish to uniformly mix; step 4, culturing: after the agar is solidified, turning over the plates, and culturing at 36 +/-1 DEG C for 48 +/-2 hours; and step 5, identifying, wherein cultured test colonies show colonies in the shape of snowflake or plum blossom. By means of the method, the raw materials containing the bacillus cereus and possibly having risks can be screened out in time within the shortest time, and the safety of jelly food is improved.