8 results about "Aspergillus niger" patented technology

The invention relates to a method for preparing sodium gluconate through adopting a semicontinuous fermentation technology. The method comprises the following steps: 1, putting a fermentation medium into a fermentation tank, accessing an Aspergillus niger spore liquid, and carrying out temperature maintenance culture; 2, discharging 20-50% of the obtained culture solution, and simultaneously supplementing the fresh medium which is isometric to the discharged culture solution to culture; 3, circulatingly carrying out step 2; and 4, discharging the obtained material from the tank. Compared withtraditional fermentation technologies, the preparation method of the invention, which adopts the fermentation medium with the sugar concentration of 5-10% higher than sugar concentrations of traditional fermentation mediums, has the advantages of improvement of the content of sodium gluconate in the total fermentation liquid, reduction of the steam disinfection frequency of the fermentation tank and a pipeline thereof, steam consumption reduction, strong production continuity, guarantee of the uniformity and the continuity of post-extraction production, and finished product quality stability guarantee.

The extraction process of plant mildew-proof preservative includes crushing lilac flower, soaking in water, distilling to obtain distillate, the first extraction of the distillate with solvent, the second extraction of the supernatant with sodium bicarbonate, the third extraction of the second supernatant with sodium bicarbonate, and volatilizing the solvent in the lower layer to obtain the plant mildew-proof preservative. The plant mildew-proof preservative has obvious and lasting inhibition on blue mold and Aspergillus niger with serious infection to cut of fruit and vegetable, has active effective period as long as 15 months, and may be used in preserve various kinds of fruits and vegetable.

The invention relates to the technical field of feed additives, in particular to a special micro-ecological preparation for laying hens and a preparation method of the special micro-ecological preparation. The special micro-ecological preparation for the laying hens comprises the following raw materials: bacillus natto powder, aspergillus niger powder, calcium tartrate and chitosan oligosaccharide, wherein the bacillus natto powder and the aspergillus niger powder are prepared by adopting a solid fermentation method, the calcium tartrate is prepared by decalcifying shrimp and crab shells, andthe chitosan oligosaccharide is prepared by taking the decalcified shrimp and crab shells as a raw material to prepare a fermentation culture medium, inoculating bacillus subtilis and paecilomyces cicadae, and carrying out fermentation degradation to obtain the special micro-ecological preparation. Laying hen feed added with the special micro-ecological preparation for the laying hens is used forfeeding the laying hens, so that the concentrated protein content of obtained eggs can be increased, meanwhile, the eggshell thickness is increased, and the quality of the eggs is remarkably improved.

The invention relates to a durable antibacterial waterborne woodenware coating and a preparation method thereof. The coating comprises the following components: water soluble alkyd resin, a waterbornepolyurethane polyol dispersion body, an amino resin mixture, nano TiO2, nano ZnO, an association type thickener, a leveling agent, a defoamer, a basic wetting agent and deionized water, wherein the amino resin mixture is composed of methylated amino resin and ketone crosslinked polyamide polyamine epichlorohydrin with the weight ratio of 1:(0.2-0.6). The waterborne coating provided by the invention has durable antibacterial activity and mildew-proof performance, has a wide antibacterial spectrum, and has the killing effect on common pathogens such as staphylococcus aureus, streptococcus pneumonia, candida albicans, pseudomonas aeruginosa and aspergillus niger reaching 99.90% or more; through SGS ASTM F1980 tests, the woodenware coating still has the bacteriostasis rate of 99.30% or more 5years later, and has 5 years or more antibacterial effect.

The invention discloses a method for improving the thermal stability of glucose oxidase, and belongs to the field of enzyme engineering. A fusion enzyme is obtained by fusing amphiphilic oligopeptideson the N end on the basis of the aspergillus-niger-sourced glucose oxidase. Compared with initial enzymes, the fusion glucose oxidase has the advantages that after the incubation for 30 min under thecondition of 60 DEG C, the residual enzyme activity is 3.8 times of the initial enzyme activity; the thermal stability of the fusion enzyme is obviously improved. By using the method, the thermal stability of glucose oxidase is effectively improved.

The invention discloses a biological organic fertilizer and production equipment of the biological organic fertilizer. The biological organic fertilizer comprises the following components in parts byweight: 40-50 parts of crop straws, 15-20 parts of biological enzyme, 40-50 parts of plant stems and leaves, 40-50 parts of plant ash, 15-20 parts of a humic agent, 6-10 parts of ammonium sulfate and6-10 parts of potassium sulfate. The humic agent is prepared from the following material components in parts by weight: 3-4 parts of aspergillus niger, 2.5-3 parts of trichoderma viride, 1.2-2 parts of trichoderma pseudokoningii, 0.8-1 part of cytophaga fermentans, 2.5-3 parts of bacillus amyloliquefaciens, 1.3-2 parts of succinic acid filamentous bacillus and 8-10 parts of chitosan. The biological organic fertilizer has the advantages that the raw materials are accessible, wastes are changed into valuable substances, the problem of environmental pollution is solved, and the fertilizer has theeffects of improving soil, enhancing fertilizer efficiency, regulating crop growth, enhancing crop stress resistance, improving crop quality and the like.