The invention discloses a garden seedling soilless planting culture fluid and its preparation method. The garden seedling soilless planting culture fluid is prepared from, by weight, 25-35 parts of methane fluid, 20-25 parts of plant straw, 11-18 parts of expanded perlite, 6-11 parts of ethylene diamine tetraacetic acid, 10-15 parts of earthworm soil, 8-15 parts of sodium silicate, 11-22 parts of rotted fig, 6-12 parts of liquorice, 8-11 parts of beta-cyclodextrin, 1-3 parts of mulberry juice, 3-5 parts of calcium nitrate, and 4-9 parts of rare earth element. The garden seedling soilless planting culture fluid is prepared by applying methane fluid, plant straw, expanded perlite, earthworm soil, and ethylene diamine tetraacetic acid as main raw materials, and adding composite vitamin, plant hormones and acidity regulator; the garden seedling soilless planting culture fluid is reasonable in formula and simple in preparation technique; the garden seedling soilless planting culture fluid is scientific in formula, safe and free from pollution; besides, the garden seedling soilless planting culture fluid has the advantages of providing various nutrition elements for flower growth, effectively solving the common nutrition loss during the growth process of distilled water hydroponics flower, and improving the quality of the distilled water hydroponics flower.