The invention relates to a preparation method of a shii-take culture medium with mulberry shoots being main raw materials, and belongs to the technical field of shii-take
culture mediums. The mulberryshoots are placed into a smashing
machine to be smashed, so that mulberry
shoot wood bits are obtained; according to a formula proportion, 65-75 wt% of the obtained mulberry
shoot wood bits, 20-30 wt% of rice
bran, 0.5-2 wt% of
calcium superphosphate, 1-3.4 wt% of
lime, 0.1-1.5 wt% of
urea and 0.5-1 wt% of white granulated
sugar are mixed, so that a total ingredient is obtained, water with the
mass being 1.2-1.5 times that of the total ingredient is added for uniform stirring, and then a culture material with pH being 6 is obtained; the obtained culture material is subjected to high-pressuresteam sterilization for 3-5 hours or normal-pressure
steam sterilization for 12-24 hours, so that the shii-take culture medium is obtained. Spawn
running time of the shii-take culture medium preparedthrough the method is short, and the
biological efficiency of shii-take is high.