5 results about "Streptomycin" patented technology

The invention discloses an improved culture medium for neonatal pig islet cells and a use method of the same. An apoptosis inhibitor Z-VAD-FMK, a human basic fibroblast growth factor hFGF-beta, a vascular endothelial growth factor VEGF, an insulin-like growth factor R3-IGF-1, a human epidermal growth factor hEGF, a hepatocyte growth factor hHGF, pig serum, sodium selenite, insulin, transferrin, ethanol amine, 1-methyl 3-isobutyl xanthine, nicotine, cortisol, penicillin and streptomycin are added in HAM'S F10 culture medium. According to the culture medium, apoptosis can be reduced and recovery rate can be increased; the cultured cells have a sensitive glucose stimulation response and more mature functions compared with generally-cultured islet cells; the insulin secretion amount of the islet cells with the same quantity is increased; the viability of islet beta-cells is improved; the breakages of islet cell clusters are reduced, and the envelops of the cell clusters are complete, so that the cell clusters are more suitable for being transplanted.

The invention discloses a culture medium for hepatoma organ cell sphere culture. The culture medium is composed of a basic culture medium, 10-25 ng/mL of an epidermal growth factor, 10-25 ng/mL of a fibroblast growth factor, 0.5-2 ng/mL of a cell growth factor, 1-3 ng/mL of an insulin growth factor, 1 mmol/L of sodium pyruvate, 2 mmol/L of glutamine, 100 U/mL of penicillin, 100 [mu]g/mL of streptomycin and 10% by volume of serum. Aiming at the culture growth characteristics of liver cancer tissue-derived cells, the culture medium provided by the invention is simple and reasonable in components, rich in nutrition and moderate in formula price; and hepatoma organ cell spheres cultured by the culture medium can effectively and stably grow for a long time, are closely gathered, have smooth andround surfaces and clear boundaries, is free of disintegration phenomenon, and can be significantly improved in cell activity, specific functions and metabolic functions.

The invention belongs to the technical field of animal breeding, and particularly relates to animal fresh semen low-temperature diluent as well as a preparation method and application thereof. The animal fresh semen low-temperature diluent is prepared from the following ingredients: glucose, sodium citrate, penicillin, streptomycin, enrofloxacin, yolk, defatted milk, vitamin A, vitamin D, vitaminE and water. The diluent has the advantages that Australian white sheep semen is stored for 1 to 2 days in a liquid state form under the condition of low temperature being 0 to 5 DEG C; the artificialinsemination requirement can be reached; the fresh semen storage quality is improved, so that the utilization rate of excellent rams is improved; high nanny goat conception rate is obtained; the problem of long-distance transportation after excellent ram semen collection is solved; the manufacturing method of the diluent is simple; the raw material cost is low; the operability is high; the large-scale popularization and application in the production is convenient.

The invention discloses a traditional Chinese medicine preparation for treating the side effects of the streptomycin. The traditional Chinese medicine preparation is prepared from the following raw materials in parts by weight: 20 to 30 g of concha haliotidis, 10 to 20 g of Chinese angelica, 10 to 20 g of divaricate saposhnikovia root, 10 to 20 g of fried bighead atractylodes rhizome, 10 to 20 g of caulis bambusae in taeniis, 10 to 20 g of rheum officinale, 10 to 20 g of chrysanthemum, 10 to 20 g of rhizoma cyperi, 10 to 20 g of fructus tribuli, 8 to 15g of ramuli et spina uncariae, 8 to 12 g of rhizoma gastrodiae, 8 to 12 g of parasitic loranthus and 8 to 12 g of cicada slough. The selected medicines are excellent in compatibility and take effects of calming liver wind, nourishing blood for tranquillization, notifying kidney and spleen and managing qi and activating blood; clinical application shows that the traditional Chinese medicine preparation disclosed by the invention can improve immunity of the organism, improve blood circulation of the organism, obviously reduce or eliminate the toxic or side effects caused by administration of streptomycin and increase the cure rate of the disease and is worthy of clinical popularization and application.