The invention discloses a biological organic fertilizer and production equipment of the biological organic fertilizer. The biological organic fertilizer comprises the following components in parts byweight: 40-50 parts of crop straws, 15-20 parts of biological enzyme, 40-50 parts of plant stems and leaves, 40-50 parts of plant ash, 15-20 parts of a humic agent, 6-10 parts of ammonium sulfate and6-10 parts of potassium sulfate. The humic agent is prepared from the following material components in parts by weight: 3-4 parts of aspergillus niger, 2.5-3 parts of trichoderma viride, 1.2-2 parts of trichoderma pseudokoningii, 0.8-1 part of cytophaga fermentans, 2.5-3 parts of bacillus amyloliquefaciens, 1.3-2 parts of succinic acid filamentous bacillus and 8-10 parts of chitosan. The biological organic fertilizer has the advantages that the raw materials are accessible, wastes are changed into valuable substances, the problem of environmental pollution is solved, and the fertilizer has theeffects of improving soil, enhancing fertilizer efficiency, regulating crop growth, enhancing crop stress resistance, improving crop quality and the like.