The invention relates to the technical field of
molecular biology, and in particular, relates to a biomarker combination for early screening of
esophageal cancer, a kit and application, wherein the biomarker combination comprises four pairs of target
specific primers, four specific primer probes, corresponding internal reference primers and internal reference primer probes which are related to early
methylation sites of
esophageal cancer people. Compared with the prior art, a plurality of pairs of PCR primers are designed by screening
esophageal cancer early
genome hypermethylation regions of
Chinese population and selecting an interval containing at least two sites in CCGC, CCGG, GCGC, ACGT and GCGG as a target spot aiming at the regions; finally, four pairs of primers and corresponding probe sequences which can distinguish tumor tissues from normal tissues under the assistance of
methylation-sensitive incision
enzyme and are high in PCR efficiency, strong in specificity and good in stability are screened out, the early esophageal
cancer detection rate is increased, the esophageal
cancer treatment effect is improved, and the esophageal
cancer death rate is reduced.