15 results about "Specific primers" patented technology

The invention discloses a quantitative detection method for FOC (Fusarium oxysporum f. sp. cubense (E.F. Smith) Snyd. & Hans.) race 4 from the soil. The method comprises the following steps: pretreating a sample to be detected and extracting DNA of the sample to be detected; preparing specific primers; conducting fluorescent nucleic acid isothermal amplification detection technique RealAmp reaction; constructing a RealAmp standard curve; and determining the result. The real-time fluorescent nucleic acid isothermal amplification detection technology provided by the invention is a novel nucleic acid detection technology combining a new generation of isothermal nucleic acid amplification technology with the real-time fluorescent detection technology, and has the advantages of high sensitivity, high specificity, low pollution and stable reaction. RealAmp can quantitatively detect pathogens; because of the characteristics of LAMP, Bst DNA polymerase has chain exchange capacity and good tolerance of inhibiting substances contained in the soil DNA extracts; the method carries out detection work on the soil, avoids cover opening and influence by contaminants and conducts on-site soil sample detection; the method for analysis and determination of reaction products is very simple; therefore, the method is suitable for wide application.

The invention discloses valid combinations of 8 novel SSR molecular markers and 2 known SSR molecular markers. The valid combinations are applied to strain identification. DNAs of 22 specific strains are taken as templates, 10 pairs of SSR primers are respectively utilized for carrying out PCR amplification, each pair of SSR primers is subjected to acrylamide gel electrophoresis after the PCR amplification, banding pattern statistic analysis is carried out according to all electrophoretic bands amplified from 22 samples, and therefore, a standard banding pattern of each pair of primers is determined; then the standard banding patterns of 10 pairs of primers of each sample are combined, and molecular identification cards of the 22 different strains of agaricus bisporus are constructed. According to an agaricus bisporus SSR molecular marker specific primer system and an application thereof, the strain identification effect of agaricus bisporus is improved; the agaricus bisporus SSR molecular marker specific primer system and the application are significant for the resource identification, the protection, the development and the utilization of agaricus bisporus.

The invention relates to a novel orthobunyavirus fluorescence quantitative RT-PCR (Reverse Transcriptase-Polymerase Chain Reaction) detection kit and a novel orthobunyavirus detection method, and aims to provide the kit which is convenient to use and accurate in detection and the detection method which is high in accuracy and high in detection efficiency. The technical scheme is that: the novel orthobunyavirus fluorescence quantitative RT-PCR detection kit comprises a specific primer, a fluorescent probe, a PCR buffer solution, dideoxyribonucleotide triphosphate and a mixed preparation, wherein an upstream primer has a sequence of FP:5'-TGGCCCTGGCTCTATAAACAT-3'; a downstream primer has a sequence of RP:5'-AATGGCAGCCCAAATGAATC-3'; the specific probe has a sequence of P:5'-FAM-TCCAATGAYGCCAAGAAGTGGAA-BHQ1-3'; FAM is a fluorescent reporter group; and BHQ1 is a fluorescent quenching group.

The invention provides a detection method for genotyping of human platelet alloantigen (HPA). The detection method is characterized in that a target fragment is obtained by designing a specific primerand then using a single-tube multiplex PCR amplification reaction; a single base extension primer is designed and then single base extension is performed; then a target DNA sequence is accurately measured by mass spectrometry. The platelet alloantigen HPA-1 to 21 bw allele can be detected at one time. Then a mass spectrum is typed directly, and data are directly output. A whole process is closed,the time is short, and the cost is low. Through detecting the genotyping of the platelet alloantigen of a patient and a blood donor, platelets matching successfully can be found, and the problem of ineffective platelet infusion caused by immune factors is effectively avoided. A detection kit product is low in cost and more accurate, and the detection method is expected to be a routine clinical detection method to replace a serological detection method.

The invention relates to primers used for amplifying plecoptera insect mitochondrion COI (cytochrome c oxidase I) gene sequences, the plecoptera insect mitochondrion COI gene sequences are as shown in SEQ ID No.1 and SEQ ID No.2. The primers for amplifying are adopted, so as to amplify COI genes of various plecoptera species effectively, be conducive to accurately identifying close species difficult to indentify in some forms, and an important tool for classification and identification, phylogeny, population genetic structures and geographical population identification of plecoptera insects in China is provided.

The invention relates to a kit, which designs a specific primer and a fluorescence labeled probe by adopting a real-time fluorescence polymerase chain reaction (PCR) technology and aiming at the conserved region of an NDM-1 pan-drug resistant gene and is used for specifically amplifying and detecting the NDM-1 pan-drug resistant gene segment. The technical scheme of the invention is to provide an NDM-1 pan-drug resistant gene PCR assay kit. The kit comprises a PCR reaction solution, wherein the PCR reaction solution comprises an upstream primer shown as SEQ ID NO.1, a downstream primer shown as SEQ ID NO.2 and a fluorescence probe shown as SEQ ID NO.3. The kit has high sensitivity and specificity, judges the result objectively and accurately, and provides a powerful tool for detecting existence of the NDM-1 pan-drug resistant gene in a clinical sample.

The invention relates to a kit for detecting whether a blood sample to be detected carries hepatitis C virus. The kit has four specific primers. In the using method for the kit, RNA extracted from the sample to be detected is taken as a template, the specific primers, polymerase, buffer and water are added into the template for loop mediated isothermal amplification (LAMP) reaction, a reaction product (or the reaction product is added with ethidium bromide) is observed under an ultraviolet lamp, and whether the sample to be detected carries the hepatitis C virus or not is judged according to a fact that whether the reaction product generates fluorescence under the ultraviolet; or the reaction product is subjected to agar gel electrophoresis, and whether the sample to be detected carries the hepatitis C virus or not is judged according to a fact that whether the reaction product has a belt in a specific track.

The invention relates to a method for rapidly detecting a pathogen of the rice black-streaked dwarf. The method comprises the following steps: performing RT-PCR amplification by using a pair of virus specific primers of a rice black-streaked dwarf virus and a southern rice black-streaked dwarf virus and by taking the total RNA of a sample to be detected as a template, and analyzing a high resolution solubility curve of an amplification product under the sealing condition, thereby judging the pathogen of the rice black-streaked dwarf. The method has the benefits that aiming at the production practice, a method for rapidly and synchronously detecting and distinguishing RBSDV from SRBSDV with high flux by using only one pair of the primers without any other probe or step of electrophoresis after amplification, EB dyeing or observation in ultraviolet lamps is developed and established, and technical support is provided for accurate diagnosis, prediction and forecasting and scientific prevention and control on the rice black-streaked dwarf.

The invention belongs to the technical field of white spirit detection, and discloses primers, a kit and an identification method for identifying bacillus amyloliquefaciens in yeast for making hard liquor. The primer for identifying the bacillus amyloliquefaciens in the yeast for making hard liquor is a pair of specific ARMS primers: ARMS-1F and ARMS-1R, and the nucleotide sequences of the ARMS-1F and the ARMS-1R are respectively shown as SEQ ID NO: 1 and SEQ ID NO: 2. A qPCR technology is utilized, an ARMS-qPCR detection method for bacillus amyloliquefaciens is established, the bacillus amyloliquefaciens and other related strains in yeast for making hard liquor are distinguished, specificity and detection limit verification shows that the method can be used for rapidly and accurately identifying, and compared with traditional morphology and physiological and biochemical identification means, the method has the advantages that errors are large, and efficiency is low, and the method can be used for rapidly and accurately identifying the bacillus amyloliquefaciens and other related strains in the yeast for making hard liquor for making hard liquor for making hard liquor for making hard liquor for making hard liquor for making hard liquor for making hard liquor for making hard liquor for making hard liquor. The method is simple and convenient to operate, quick in reaction and capable of realizing accurate qualitative identification.

The invention discloses a method for synchronously detecting five important virus pathogens in ranunculus asiaticus. The five important virus pathogens are ranunculus mild mosaic virus, ranunculus latent virus, ranunculus asiaticus white mottle virus, ranunculus asiaticus mosaic virus and ranunculus leaf distortion virus. According to the application of the method, plant total RNA or virus RNA isextracted, different types of virus cDNA first chains are synthesized by random primers, five viruses are subjected to multiplex PCR detection by specific primers, the steps of synthesizing the cDNA first chains by reverse transcription of a single virus primer, determining and analyzing a virus nucleotide sequence and the like are omitted, a target fragment is obtained by utilizing the rapidity and sensitivity of multiplex PCR, and a scientific method is established for detecting various ranunculus asiaticus virus disease samples quickly, accurately and sensitively in quantity.

The invention discloses a detection method for genotype detection on CYP2C9 site and a kit thereof, and relates to the field of molecular biology and medicine, wherein the method is used for predicting the risk of hepatotoxicity side effect on a patient suffering gout or hyperuricemia after the patient takes benzbromarone, through genotype detection on CYP2C9 site; a specific primer and a probe are designed, which are subjected to a hybridization reaction with a MagPlex-TAG magnetic bead with specific reporter gene; the detection is carried out on an luminex 200 apparatus through a chromogenicreaction. The detection kit disclosed by the invention realizes sensitive and quick detection.