The invention belongs to the technical field of pesticides, and particularly relates to a bactericidal composition of prothioconazole and flutriafol for preventing and curing wheat stripe rust. The bactericidal composition is prepared from, by weight, 5%-50% of active ingredient, 2%-6% of wetting dispersant, 0.1%-0.4% of thickening agent, 3%-5% of antifreezing agent, 0.4%-0.6% of defoaming agent and balance water; the active ingredient is a mixture of the prothioconazole and flutriafol, the weight ratio of the prothioconazole to the flutriafol is (1-4): (1-4). The prothioconazole is a systemicprothioconazole sterilizing agent, the lutriafol is a broad-spectrum triazole fungicide, the prothioconazole and the flutriafol are mixed, do not contradict each other, and can achieve a synergisticeffect, that is, the activity of the mixture is greater than that of the single component, the dosage is reduced, the resistance is slowed down, and the cost is reduced.