The invention discloses a method for detecting
biogenic amine in yellow
wine by using a chromatographic sheet, belonging to the technical field of analysis and detection of components in the yellow
wine. The method comprises the steps of carrying out derivation on a
biogenic amine standard substance and a to-be-tested yellow
wine sample by using a
derivatization reagent, enriching the
biogenic amine by using liquid-liquid extraction, carrying out sample application on a sheet plate, arranging the sheet plate in a saturate tank loaded with a certain proportion of developing
solvent and unfolding; developing and observing under an
ultraviolet lamp, and qualitatively detecting the target biogenic amine; and finally, quantitatively measuring the biogenic amine in the yellow wine by using a
thin layer chromatograph. According to the method, the
detection limit range of the biogenic amine is 0.5-5 mug / mL, the average adding standard
recovery rate is 88.5-110.3 percent, the standard deviation is 3.8-12.5 percent, and at most 13 samples can be simultaneously detected on a 20*20
thin layer glass plate. Compared with other analysis methods, the method has the advantages that qualitative and quantitative analysis of the biogenic amine in the yellow wine can be realized, the operation is simple and convenient, the cost is low, and a plurality of samples can be simultaneously detected.