The invention provides a composition for improving the plasticity retention rate of standard gel and a preparation method and application thereof.The composition is prepared from, by weight, 40-60 parts of an agent M, 1-3 parts of span 80, 0.5-1.5 parts of Tween 80, 0.3-1 part of peregal 0, 2-3 parts of lauryl sodium sulfate and 80-100 parts of water, and the agent M is prepared from styrenated phenol, 2, 6-butylated hydroxytoluene, N-(1, 2, 4-trimethyl-1, 3-pentanediol) and N-(1, 2, 4-trimethyl-1, 3-pentanediol. According to the present invention, the prepared composition can effectively improve the plasticity retention rate of the standard gel, the plasticity initial value P0 is 31.0-38.0, the plasticity retention rate PRI is improved to 63.1-67.6, the composition is applied to the dry stirring line or waterline preparation standard gel process, the addition amount of the composition can be effectively controlled, the production cost can be reduced, the production efficiency can be improved, and the production cost can be reduced. The plasticity retention rate is improved under the condition that the mechanical property is not changed, the difficulty of a plastication procedure and the production energy consumption in rubber product production are reduced, and the stability of a rubber production process formula and the performance is facilitated.