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12 results about "Chromatography column" patented technology

A Chromatography column is a device used in chromatography for the separation of chemical compounds. A chromatography column contains the stationary phase, allowing the mobile phase to pass through it. Chromatography columns of different types are used in both gas and liquid chromatography.

Serum specificity metabolite spectrum for patient with lung cancer, and building method thereof

ActiveCN103616450AEasy to handleStrong qualitative analysis abilityComponent separationGas phaseOriginal data
The invention discloses a serum specificity metabolite spectrum for a patient with a lung cancer and a building method thereof, and particularly discloses a method for carrying out liquid-phase/gas-phase chromatography mass spectrometry metabonomics analysis based on the patient suffering from the lung cancer. The method comprises the following steps: collecting fasting blood samples of a healthy and normal detected person and the patient suffering from the lung cancer 7 days before and after an operation, separating by a chromatogram column after processing; extracting and aligning original data of an instrument after adopting mass spectrometry analysis; building a mathematical model to analyze the metabolites spectrum by adopting multivariable data statistical software; comparing with the difference between an average person with the metabolites spectrum of the patient with the lung cancer, so as to determine the specificity metabolite spectrum. By adopting the method disclosed by the invention, the mutation status of the metabolite of the patient with the lung cancer can be generally and comprehensively reflected, and the serum specificity metabolite spectrum can be applied to early diagnosis of the lung cancer or a beneficial technical support is supplied for prognosis.
Owner:HUZHOU CENT HOSPITAL

Valine purification method

ActiveCN101798273AEasy to operateGood choiceIon-exchange process apparatusIon-exchanger regenerationPurification methodsFiltration
The invention relates to a valine purification method, which comprises the following steps: A. adding flocculating agents into valine fermentation liquid for fast precipitating solid impurities, and obtaining primary filter liquid through filtering supernatant; B. adding activated carbon into the primary filter liquid obtained in the step A, wherein the added activated carbon accounts for 1+ / -0.5weight percent of the filter liquid, carrying out stirring and decoloration at 60 + / - 5 DEG C, and then, obtaining secondary filter liquid through filtration; C. using an industrial chromatographic column for separating inorganic salt and heteroacid, and then, using a sodium filter membrane for removing pigment and small molecular impurities to obtain a clear and colorless valine solution; D. concentrating the filter liquid for crystallization to obtain crude valine products; and E. shaking a transmission belt and raising the temperature to 220 + / - 10 DEG C, and carrying out sublimation to remove alanine and isoleucine to obtain pharmaceutical grade competitive valine products. The method has the characteristics of simple operation, good selectivity, cleanness and environment protection, so the yield and the quality of the valine are obviously improved.
Owner:ZHAODONG XINGHU BIOTECHNOLOGY CO LTD

Blood disease related drug concentration detection method and application

PendingCN110940742AComponent separationTriple quadrupole mass spectrometryHematologic disease
The invention relates to a blood disease related drug concentration detection method and application, and the method comprises the following steps: mixing a blood sample to be detected with a cell lysis solution or a buffer salt solution, adding a methanol solution of an isotope internal standard of the drug to be detected, oscillating, carrying out centrifugal separation, and collecting the supernatant; injecting the supernatant into a rapid liquid chromatography system for separation and analysis, then entering a triple quadrupole mass spectrometry detector, collecting and processing data through chromatographic software, and calculating the drug concentration in a blood sample to be detected. According to the blood disease related drug concentration detection method, according to the characteristics and clinical needs of the blood diseases, a scheme for detecting the blood concentration of the medicines related to the blood diseases is established, and the scheme has the advantagesthat a chromatographic column and a mobile phase do not need to be replaced, so that the detection efficiency and the operation convenience are greatly improved, and the method is suitable for large-scale clinical popularization.
Owner:BEIJING LU DAOPEI BIOTECHNOLOGY CO LTD

Sample pre-compression valve for liquid chromatography

ActiveUS20180306758A1To offer comfortComponent separationFluid phasePhysical chemistry
A sample pre-compression valve for liquid chromatography applications is described. The valve enables a sample pre-compression while the solvent pump continues to conduct solvent to the chromatography column. Furthermore, the sample pre-compression valve includes an INJECT position, a LOAD position and a PUMP PURGE position, in which all connecting grooves of the valve are flushed with liquid. A use of the sample pre-compression valve is described as part of a sampler for liquid chromatography applications.
Owner:DIONEX SOFTRON

Tylonolide content detection method

ActiveCN104569196AHigh precisionImprove accuracyComponent separationColumn temperatureAcetonitrile
The invention provides a Tylonolide content detection method. Analysis is performed with an Agilent-1260 high performance liquid chromatograph, a DAD (diode array detector) is used, the detection wavelength is 216 nm, the sample size is 20 mu l, and the chromatographic condition is as follows: a chromatographic column takes octadecylsilane chemically bonded silica as filler, and an Agilent-1260 high performance liquid chromatography Wondasil C18 column is used for determination; the mobile phase is acetonitrile-0.5% formic acid, and the volume fraction of acetonitrile is in a range of 65%-80%; the flow velocity is in a range of 0.6-1.5 ml / min; the column temperature is in a range of 20-40 DEG C, automatic sample injection is performed on a standard substance and a to-be-tested substance respectively, and the Tylonolide content is calculated by the aid of the peak area with an external standard method; the Tylonolide content detection method is simple, convenient, good in separation effect and high in precision and accuracy.
Owner:TIANJIN ZHONGSHENG TIAOZHAN BIOTECH

Method for decolorizing tremella root polysaccharide

PendingCN110105464ALow viscosityHigh decolorization rateTremellaPolyamide
The invention relates to polysaccharide, in particular to a method for decolorizing tremella root polysaccharide. The method comprises the following steps of S1, extraction of root crude polysaccharide for standby application; S2, purification of the root crude polysaccharide, wherein the obtained root crude polysaccharide is subjected to chromatography by a polyamide resin chromatography column to obtain a polyamide purified polysaccharide solution; S3, secondary purification, wherein the obtained polyamide purified polysaccharide solution is added into HP-20 macroporous resin for adsorption,and centrifugal separation is carried out to obtain a root decolorization polysaccharide solution; S4, concentration and drying, wherein the obtained root decolorization polysaccharide solution is subjected to decompression concentration and vacuum freezing in sequence to obtain the tremella root polysaccharide. The method for decolorizing the tremella root polysaccharide has the advantage of improving the decolourization rate of the tremella root polysaccharide by using a double standing adsorption method of polyamide combined with the macroporous resin.
Owner:INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI

Six-way valve structure for online monitoring and detection of oil gas chromatogram and application method thereof

The invention relates to a six-way valve structure for online monitoring and detection of oil gas chromatogram and an application method thereof. The six-way valve structure comprises a valve body, wherein a pipe orifice A, a pipe orifice B, a pipe orifice C, a pipe orifice D, a pipe orifice E and a pipe orifice F are formed in the valve body; the pipe orifice A and the pipe orifice B are communicated through a pipeline built in the valve body; the pipe orifice C and the pipe orifice D are communicated through a pipeline built in the valve body; the pipe orifice E and the pipe orifice F are communicated through a pipeline built in the valve body; the output ends of the pipe orifice D and the pipe orifice A are communicated through a switch pipeline built in the valve body; a miniature electromagnetic valve A is arranged on the switch pipeline; a miniature electromagnetic valve B is arranged at an the input end of the pipe orifice A; the pipe orifice C is connected with the pipe orificeF through a quantifying pipe built outside; the pipe orifice A is a carrier gas inlet; the pipe orifice E is a sample inlet; the pipe orifice B is connected with a chromatographic column. The six-wayvalve structure provided by the invention has the advantages of simple structure, reasonable design and high operation quality, improves the stability of an oil chromatogram online monitoring system,and reduces the maintenance frequency; stable operation of the equipment can better reflect internal conditions of a transformer.
Owner:福建和盛高科技产业有限公司

Separation and purification method for uridine triphosphate

ActiveCN109503687AMeet high purity requirementsHigh purification yieldSugar derivativesSugar derivatives preparationPurification methodsMicrosphere
The invention provides a separation and purification method for uridine triphosphate. The separation and purification method comprises the following steps: 1) loading a sample: filtering a crude product of uridine triphosphate and then loading into a chromatographic column, wherein chromatographic column filler is made of monodisperse microspheres taking polyacrylate as substrate; 2) eluting: using an eluant for eluting UTP absorbed in the chromatographic column; 3) collecting and gathering: fractionally collecting eluted uridine triphosphate solution and gathering the component liquid meetingthe requirement for target peak. The separation and purification method for uridine triphosphate provided by the invention is simple and convenient and is short in purification period; the requirement for high purity of uridine triphosphate can be met by only one-step chromatographic purification; purity can reach up to 99% or above; purification yield is high and stable.
Owner:SUZHOU NANOMICRO TECH CO LTD

Detection method for content of chlorogenic acid in stauntonia chinensis DC

InactiveCN110133138AGood linear relationshipAvoid recycling operationsComponent separationChlorogenic acidPhosphate
The invention discloses a detection method for the content of chlorogenic acid in stauntonia chinensis DC. The detection method comprises the following steps that: 1) smashing a stauntonia chinensis DC product into powder, adding methyl alcohol, carrying out ultrasonic extraction for 30min, and filtering to obtain a sample to be detected; 2) weighing a chlorogenic acid standard product, then configuring 1mg / ml of chlorogenic acid reference substance stock solution by the methyl alcohol; 3) measuring by high performance liquid chromatography for measurement, wherein a stationary phase is an Accucore aQ chromatographic column, a moving phase A is phosphate buffered solution, a moving phase B is acetonitrile, the volume ratio of A to B is 85:15, a flow rate is 0.6mL / min, detection wavelengthis 325nm, a column temperature is 30DEG CC, and a sample feeding amount is 2[Mu]L; and 4) taking the peak area of the reference substance stock solution is a vertical coordinate, taking the concentration of the reference substance stock solution as an abscissa, drawing the standard curve of the chlorogenic acid, and calculating the content of the chlorogenic acid in the stauntonia chinensis DC ina sample to be detected. By use of the above method, the stauntonia chinensis DC product which contains the chlorogenic acid under low concentration can be effectively detected, detection time is short, and accuracy is high.
Owner:GUIZHOU TIANLOU BIOLOGICAL DEV CO LTD

Liquid chromatographic column pipe body grinding device

ActiveCN113478330AEasy to placeEasy height adjustmentEdge grinding machinesRevolution surface grinding machinesStructural engineeringMachine
The invention discloses a liquid chromatographic column pipe body grinding device which comprises a machine body and a bearing frame. Supporting legs are arranged on the peripheral wall bodies of the bottom of the machine body correspondingly, an electric control box is arranged on the wall body of the front end of one side of the top of the machine body, and a machine box is arranged on the wall body located behind the electric control box and at the top of the machine body; and a transmission box is arranged on the wall body of the rear end of the machine box and at the top of the machine body, a grinding wheel is arranged on a machine shaft at the rear end of the transmission box, a protective cover is further arranged on the wall body of the rear end of the transmission box, a waste residue pipe is arranged in the wall body of the back of the protective cover, and a sliding table is arranged on the wall body of the other side of the top of the machine body. According to the liquid chromatographic column pipe body grinding device, through the arrangement of a containing mechanism, a ground pipe body can be conveniently contained; and meanwhile, through the arrangement of a first fixing frame, a second fixing frame and a second adjusting shaft, the height of arc-shaped covers can be conveniently adjusted. Through the arrangement of a first adjusting shaft, a first sliding block and a second sliding block, the distance between the arc-shaped covers can be adjusted conveniently.
Owner:南通江涌投资开发有限公司
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